Shun Chen1, An-Chun Cheng, Ming-Shu Wang, Xi Peng. 1. Avian Diseases Research Centre, College of Veterinary Medicine of Sichuan Agricultural University, Yaan 625014, Sichuan Province, China.
Abstract
AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non-apoptotic cells decreased. CONCLUSION: NGVEV can induce the infected DEF cells to undergo apoptosis and the apoptosis occurs prior to necrosis.
AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluoresceinannexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non-apoptotic cells decreased. CONCLUSION: NGVEV can induce the infected DEF cells to undergo apoptosis and the apoptosis occurs prior to necrosis.
Authors: Valentina Rapozzi; Greta Varchi; Emilia Della Pietra; Claudia Ferroni; Luigi E Xodo Journal: Invest New Drugs Date: 2016-10-11 Impact factor: 3.850
Authors: Oscar A Lenis-Rojas; Beatriz Carvalho; Rui Cabral; Margarida Silva; Sofia Friães; Catarina Roma-Rodrigues; Marta S H Meireles; Clara S B Gomes; Jhonathan A A Fernández; Sabela F Vila; Juan A Rubiolo; Laura Sanchez; Pedro V Baptista; Alexandra R Fernandes; Beatriz Royo Journal: J Biol Inorg Chem Date: 2021-10-29 Impact factor: 3.358