| Literature DB >> 18407351 |
J Edelmann1, L Klein-Hitpass, A Carpinteiro, A Führer, L Sellmann, S Stilgenbauer, U Dührsen, J Dürig.
Abstract
Microarray-based gene expression profiling (GEP) was used to study how stroma modulates the survival of CLL cells in an in vitro coculture model employing the murine fibroblast cell line M2-10B4. CLL cells cultured in direct contact with the stromal layer (STR) showed a significantly better survival than cells cultured in transwell (TW) inserts above the M2-10B4 cells. STR as compared to TW conditions induced a significant up-regulation of PI3K/NF-kappaB pro-survival pathway genes and mediated a pro-angiogenetic switch in the CLL cells by up-regulation of vascular endothelial growth factor (VEGF) and osteopontin (OPN) and down-regulation of the anti-angiogenetic molecule thrombospondin-1 (TSP-1).Entities:
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Year: 2008 PMID： 18407351 DOI： 10.1016/j.leukres.2008.03.003
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156