| Literature DB >> 18406529 |
Hiroyuki Kabayama1, Naoko Tokushige, Makoto Takeuchi, Katsuhiko Mikoshiba.
Abstract
Neurite outgrowth is crucial for neural circuit formation. Intracellular membrane trafficking is involved in the cell surface expansion that is necessary for neurite outgrowth. It is known that syntaxin 6 is predominantly located in the Golgi region in undifferentiated PC12 cells and that it regulates trans-Golgi network trafficking and the secretory pathway via its coiled-coil domains. However, whether it also regulates neurite outgrowth remains unknown. In this paper, we found that syntaxin 6 was located both in the Golgi apparatus and the distal tips of the neurites of nerve growth factor (NGF)-treated PC12 cells. We also showed that the overexpression of the first coiled-coil domain of syntaxin 6 inhibited NGF-dependent neurite outgrowth. However, the coiled-coil domain-disrupting mutant had little effect on neurite outgrowth. These results suggest that the first coiled-coil domain of syntaxin 6 plays a crucial role in NGF-dependent neurite outgrowth.Entities:
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Year: 2008 PMID： 18406529 DOI： 10.1016/j.neulet.2008.03.061
Source DB: PubMed Journal: Neurosci Lett ISSN： 0304-3940 Impact factor: 3.046