UNLABELLED: Virtually no information is available on glucose fluxes during a meal or glucose ingestion in adolescents. AIM: To use a triple tracer approach to measure rates of appearance of ingested glucose (Ra(ogtt)), endogenous glucose production (EGP) and glucose disappearance (Rd) following an oral glucose bolus in adolescents. METHODS: Eleven adolescents (4 M/7 F, 15 +/- 1 yr; 67.3 +/- 4.7 kg; 24 +/-2 kg/m2) underwent a frequent sampled oral glucose tolerance test (OGTT) (labelled with [6,6-2H2]glucose) combined with intravenous infusion of [1-(13)C]glucose and [U-(13)C6]glucose following an overnight fast. Formulas were developed to estimate glucose fluxes using one- or two-compartment models. RESULTS: During the 7 h following the OGTT bolus, 9.8 +/- 2.3% of the ingested glucose was extracted by the liver, EGP was suppressed by 45 +/- 4% and Rd increased by 21 +/- 5%. CONCLUSIONS: The triple tracer method provided accurate assessment of Ra(ogtt), EGP and Rd fluxes during an OGTT in adolescents. Thus, this method might provide novel insight on postprandial glucose fluxes in children/adolescents under various conditions.
UNLABELLED: Virtually no information is available on glucose fluxes during a meal or glucose ingestion in adolescents. AIM: To use a triple tracer approach to measure rates of appearance of ingested glucose (Ra(ogtt)), endogenous glucose production (EGP) and glucose disappearance (Rd) following an oral glucose bolus in adolescents. METHODS: Eleven adolescents (4 M/7 F, 15 +/- 1 yr; 67.3 +/- 4.7 kg; 24 +/-2 kg/m2) underwent a frequent sampled oral glucose tolerance test (OGTT) (labelled with [6,6-2H2]glucose) combined with intravenous infusion of [1-(13)C]glucose and [U-(13)C6]glucose following an overnight fast. Formulas were developed to estimate glucose fluxes using one- or two-compartment models. RESULTS: During the 7 h following the OGTT bolus, 9.8 +/- 2.3% of the ingested glucose was extracted by the liver, EGP was suppressed by 45 +/- 4% and Rd increased by 21 +/- 5%. CONCLUSIONS: The triple tracer method provided accurate assessment of Ra(ogtt), EGP and Rd fluxes during an OGTT in adolescents. Thus, this method might provide novel insight on postprandial glucose fluxes in children/adolescents under various conditions.
Authors: D Elleri; J M Allen; J Harris; K Kumareswaran; M Nodale; L Leelarathna; C L Acerini; A Haidar; M E Wilinska; N Jackson; A M Umpleby; M L Evans; D B Dunger; R Hovorka Journal: Diabetologia Date: 2013-02-23 Impact factor: 10.122
Authors: Fernando García-García; Roman Hovorka; Malgorzata E Wilinska; Daniela Elleri; M Elena Hernando Journal: Med Biol Eng Comput Date: 2016-05-07 Impact factor: 2.602
Authors: Claudio Cobelli; Chiara Dalla Man; Giovanni Sparacino; Lalo Magni; Giuseppe De Nicolao; Boris P Kovatchev Journal: IEEE Rev Biomed Eng Date: 2009-01-01