| Literature DB >> 184049 |
Abstract
An assay for bovine interferons has been developed using the porcine cell line IB-RS-2 and a bovine enterovirus, CBV-D, as challenge virus. The method is based on estimation of cytopathic effect measured by uptake of neutral red. Teh assay is simple, sensitive, and reproducible. A comparative test of different viruses in IB-RS-2 cells and secondary calf kidney cells revealed that the sensitivity of a virus to interferon can vary up to 1,000-fold in the two cell systems. Vesicular stomatitis virus was found to be rather insensitive to interferon in IB-RS-2 cells.Entities:
Mesh:
Substances:
Year: 1976 PMID: 184049 PMCID: PMC420927 DOI: 10.1128/iai.14.3.603-606.1976
Source DB: PubMed Journal: Infect Immun ISSN: 0019-9567 Impact factor: 3.441