| Literature DB >> 18400430 |
Yan Zhuang1, Jingjing Yan, Wei Zhu, Lirong Chen, Dehai Liang, Xiaojie Xu.
Abstract
"Frequent hitter" phenomenon emerged in the high-throughput screening; one of the most common mechanisms behind artifactual inhibition is that some organic molecules formed large colloid-like aggregates which are able to sequester and thereby inhibit enzymes. To investigate the situation in Traditional Chinese Medicine (TCM), 60 medicinal herbs and 24 Chinese herbal formulae were detected by dynamic light scattering (DLS), and aggregates were observed in all the 84 solution mixtures. The aggregates of two Chinese herbal formulae, 'Xue-Fu-Zhu-Yu Tang' (XF) and 'Jing-Guan Tang' (JG), were not only able to survive in the gastro-intestinal environment, but also had the ability to pass through the monolayer of the Caco-2 cell. The activities of XF and JG against three cardiovascular targets were also aggregates-related. Based on these findings, a new possible mechanism of the action of Chinese medicine was proposed.Mesh：
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Year: 2008 PMID： 18400430 DOI： 10.1016/j.jep.2008.02.017
Source DB: PubMed Journal: J Ethnopharmacol ISSN： 0378-8741 Impact factor: 4.360