Literature DB >> 18398685

Internal pH indicators for biomolecular NMR.

Olga K Baryshnikova1, Thomas C Williams, Brian D Sykes.   

Abstract

We describe a non-invasive technique for determining pH in biomolecular NMR sample using buffer components (formate, tris, piperazine, and imidazole) as internal pH indicators, whose (1)H NMR chemical shifts are sensitive to pH in a range from 2.5 to 9.8. This method is suitable for a wide range of applications where samples are handled intensively during NMR titrations or in high throughput analysis in structural genomics or metabolomics.

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Year:  2008        PMID： 18398685     DOI： 10.1007/s10858-008-9234-6

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  9 in total

1.  Structure of cardiac muscle troponin C unexpectedly reveals a closed regulatory domain.

Authors:  S K Sia; M X Li; L Spyracopoulos; S M Gagné; W Liu; J A Putkey; B D Sykes
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2.  Spectroscopic measurement of a long-predicted active site pK in iron-superoxide dismutase from Escherichia coli.

Authors:  D L Sorkin; A F Miller
Journal:  Biochemistry       Date:  1997-04-22       Impact factor: 3.162

3.  Proton nuclear magnetic resonance spectroscopy of aqueous solutions: complete elimination of the water resonance by spin-spin relaxation.

Authors:  D L Rabenstein; S Y Fan
Journal:  Anal Chem       Date:  1986-12       Impact factor: 6.986

4.  alpha - and beta -phosphorylated amines and pyrrolidines, a new class of low toxic highly sensitive 31P NMR pH indicators. Modeling of pKa and chemical shift values as a function of substituents.

Authors:  S Pietri; M Miollan; S Martel; F Le Moigne; B Blaive; M Culcasi
Journal:  J Biol Chem       Date:  2000-06-30       Impact factor: 5.157

5.  19F-nuclear magnetic resonance: measurements of [O2] and pH in biological systems.

Authors:  J Taylor; C Deutsch
Journal:  Biophys J       Date:  1988-02       Impact factor: 4.033

6.  Binding of cardiac troponin-I147-163 induces a structural opening in human cardiac troponin-C.

Authors:  M X Li; L Spyracopoulos; B D Sykes
Journal:  Biochemistry       Date:  1999-06-29       Impact factor: 3.162

7.  Use of diethyl(2-methylpyrrolidin-2-yl)phosphonate as a highly sensitive extra- and intracellular 31P NMR pH indicator in isolated organs. Direct NMR evidence of acidic compartments in the ischemic and reperfused rat liver.

Authors:  S Pietri; S Martel; M Culcasi; M C Delmas-Beauvieux; P Canioni; J L Gallis
Journal:  J Biol Chem       Date:  2000-09-29       Impact factor: 5.157

8.  Structure and dynamics of the C-domain of human cardiac troponin C in complex with the inhibitory region of human cardiac troponin I.

Authors:  Darrin A Lindhout; Brian D Sykes
Journal:  J Biol Chem       Date:  2003-05-05       Impact factor: 5.157

9.  Characterization of methylphosphonate as a 31P NMR pH indicator.

Authors:  M DeFronzo; R J Gillies
Journal:  J Biol Chem       Date:  1987-08-15       Impact factor: 5.157
  9 in total
  26 in total

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3.  Rapid and efficient purification of Drosophila homeodomain transcription factors for biophysical characterization.

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Journal:  Protein Expr Purif       Date:  2019-02-07       Impact factor: 1.650

4.  Structure of phosphorylated UBL domain and insights into PINK1-orchestrated parkin activation.

Authors:  Jacob D Aguirre; Karen M Dunkerley; Pascal Mercier; Gary S Shaw
Journal:  Proc Natl Acad Sci U S A       Date:  2016-12-22       Impact factor: 11.205

5.  Elucidation of isoform-dependent pH sensitivity of troponin i by NMR spectroscopy.

Authors:  Ian M Robertson; Peter C Holmes; Monica X Li; Sandra E Pineda-Sanabria; Olga K Baryshnikova; Brian D Sykes
Journal:  J Biol Chem       Date:  2011-12-17       Impact factor: 5.157

6.  The effect of the cosolvent trifluoroethanol on a tryptophan side chain orientation in the hydrophobic core of troponin C.

Authors:  Olivier Julien; Pascal Mercier; Melissa L Crane; Brian D Sykes
Journal:  Protein Sci       Date:  2009-06       Impact factor: 6.725

7.  Electrostatic Energetics of Bacillus subtilis Ribonuclease P Protein Determined by Nuclear Magnetic Resonance-Based Histidine pKa Measurements.

Authors:  Pamela L Mosley; Kyle G Daniels; Terrence G Oas
Journal:  Biochemistry       Date:  2015-08-20       Impact factor: 3.162

8.  The cardiac-specific N-terminal region of troponin I positions the regulatory domain of troponin C.

Authors:  Peter M Hwang; Fangze Cai; Sandra E Pineda-Sanabria; David C Corson; Brian D Sykes
Journal:  Proc Natl Acad Sci U S A       Date:  2014-09-22       Impact factor: 11.205

9.  β-amyloid model core peptides: Effects of hydrophobes and disulfides.

Authors:  Laura M L Hawk; Jay M Pittman; Patrick C Moore; Atul K Srivastava; Jonathan Zerweck; Joshua T B Williams; Andrew J Hawk; Joseph R Sachleben; Stephen C Meredith
Journal:  Protein Sci       Date:  2019-11-25       Impact factor: 6.725

10.  The Mechanism of HdeA Unfolding and Chaperone Activation.

Authors:  Loïc Salmon; Frederick Stull; Sabrina Sayle; Claire Cato; Şerife Akgül; Linda Foit; Logan S Ahlstrom; Elan Z Eisenmesser; Hashim M Al-Hashimi; James C A Bardwell; Scott Horowitz
Journal:  J Mol Biol       Date:  2017-11-11       Impact factor: 5.469
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