Literature DB >> 1839500

Screening recombinant DNA libraries: a rapid and efficient method for isolating cDNA clones utilizing the PCR.

N R Isola1, H J Harn, D L Cooper.   

Abstract

We describe an expeditious method for the isolation of cDNA clones utilizing PCR-based amplification of target sequences from cDNA libraries. This method is rapid, less labor-intensive and inexpensive when compared with screening libraries with radiolabeled probes. This method can be applied to isolate multiple members of a protein family as well as homologous genes in different species by designing appropriate primers to amplify the most conserved regions. Utilizing this method, a novel reticulocyte CD44 transcript was isolated.

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Year:  1991        PMID： 1839500

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  4 in total

Review 1.  CD44 in cancer progression: adhesion, migration and growth regulation.

Authors:  R Marhaba; M Zöller
Journal:  J Mol Histol       Date:  2004-03       Impact factor: 2.611

Review 2.  CD44: physiological expression of distinct isoforms as evidence for organ-specific metastasis formation.

Authors:  M Zöller
Journal:  J Mol Med (Berl)       Date:  1995-09       Impact factor: 4.599

3.  Characterization of PDH beta 1, the structural gene for the pyruvate dehydrogenase beta subunit from Saccharomyces cerevisiae.

Authors:  S G Miran; J E Lawson; L J Reed
Journal:  Proc Natl Acad Sci U S A       Date:  1993-02-15       Impact factor: 11.205

4.  Structure of the rat p53 tumor suppressor gene.

Authors:  J E Hulla; R P Schneider
Journal:  Nucleic Acids Res       Date:  1993-02-11       Impact factor: 16.971
  4 in total

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