Literature DB >> 18392958

Transposon-based strategies for the identification of essential bacterial genes.

William S Reznikoff1, Kelly M Winterberg.   

Abstract

We present a conceptual review of transposition-based strategies for determining gene essentiality on a one-by-one basis in bacteria. Many of the techniques are described in greater detail in individual chapters of this volume. The second section of this chapter deals with transposition-deletion-based strategies for determining the essentiality of blocks of genes. This latter approach has the potential to experimentally define the minimal required genome for a given organism.

Mesh：

Substances：

Year:  2008        PMID： 18392958     DOI： 10.1007/978-1-59745-321-9_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Evolutionary conservation of essential and highly expressed genes in Pseudomonas aeruginosa.

Authors:  Andreas Dötsch; Frank Klawonn; Michael Jarek; Maren Scharfe; Helmut Blöcker; Susanne Häussler
Journal:  BMC Genomics       Date:  2010-04-09       Impact factor: 3.969

Review 2.  High-throughput bacterial functional genomics in the sequencing era.

Authors:  Andrew N Gray; Byoung-Mo Koo; Anthony L Shiver; Jason M Peters; Hendrik Osadnik; Carol A Gross
Journal:  Curr Opin Microbiol       Date:  2015-09-01       Impact factor: 7.934

3.  A shotgun antisense approach to the identification of novel essential genes in Pseudomonas aeruginosa.

Authors:  Ruggero Rusmini; Davide Vecchietti; Raffaella Macchi; Faustino Vidal-Aroca; Giovanni Bertoni
Journal:  BMC Microbiol       Date:  2014-02-05       Impact factor: 3.605

4.  Construction and analysis of a modified transposable element carrying an outward directed inducible promoter for Bacillus subtilis.

Authors:  Kelly Cristina Leite Mulder; Wolfgang Schumann
Journal:  Curr Microbiol       Date:  2013-12-27       Impact factor: 2.188

Review 5.  Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.

Authors:  Andrew Robinson; Rebecca J Causer; Nicholas E Dixon
Journal:  Curr Drug Targets       Date:  2012-03       Impact factor: 3.465

Review 6.  Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.

Authors:  George Silva; Laurent Poirot; Roman Galetto; Julianne Smith; Guillermo Montoya; Philippe Duchateau; Frédéric Pâques
Journal:  Curr Gene Ther       Date:  2011-02       Impact factor: 4.391

7.  Essential gene prediction using limited gene essentiality information-An integrative semi-supervised machine learning strategy.

Authors:  Sutanu Nandi; Piyali Ganguli; Ram Rup Sarkar
Journal:  PLoS One       Date:  2020-11-30       Impact factor: 3.240

8.  Identification of essential genes of the periodontal pathogen Porphyromonas gingivalis.

Authors:  Brian A Klein; Elizabeth L Tenorio; David W Lazinski; Andrew Camilli; Margaret J Duncan; Linden T Hu
Journal:  BMC Genomics       Date:  2012-10-31       Impact factor: 3.969

9.  Transposon mutagenesis in Bifidobacterium breve: construction and characterization of a Tn5 transposon mutant library for Bifidobacterium breve UCC2003.

Authors:  Lorena Ruiz; Mary O'Connell Motherway; Noreen Lanigan; Douwe van Sinderen
Journal:  PLoS One       Date:  2013-05-30       Impact factor: 3.240

10.  Harnessing the power of transposon mutagenesis for antibacterial target identification and evaluation.

Authors:  Timothy C Meredith; Hao Wang; Patrick Beaulieu; Angelika Gründling; Terry Roemer
Journal:  Mob Genet Elements       Date:  2012-07-01
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.