OBJECTIVES: The aim of this study was to determine and compare the proteomes of three triclosan-resistant mutants of Salmonella enterica serovar Typhimurium in order to identify proteins involved in triclosan resistance. METHODS: The proteomes of three distinct but isogenic triclosan-resistant mutants were determined using two-dimensional liquid chromatography mass separation. Bioinformatics was then used to identify and quantify tryptic peptides in order to determine protein expression. RESULTS: Proteomic analysis of the triclosan-resistant mutants identified a common set of proteins involved in production of pyruvate or fatty acid with differential expression in all mutants, but also demonstrated specific patterns of expression associated with each phenotype. CONCLUSIONS: These data show that triclosan resistance can occur via distinct pathways in Salmonella, and demonstrate a novel triclosan resistance network that is likely to have relevance to other pathogenic bacteria subject to triclosan exposure and may provide new targets for development of antimicrobial agents.
OBJECTIVES: The aim of this study was to determine and compare the proteomes of three triclosan-resistant mutants of Salmonella enterica serovar Typhimurium in order to identify proteins involved in triclosan resistance. METHODS: The proteomes of three distinct but isogenic triclosan-resistant mutants were determined using two-dimensional liquid chromatography mass separation. Bioinformatics was then used to identify and quantify tryptic peptides in order to determine protein expression. RESULTS: Proteomic analysis of the triclosan-resistant mutants identified a common set of proteins involved in production of pyruvate or fatty acid with differential expression in all mutants, but also demonstrated specific patterns of expression associated with each phenotype. CONCLUSIONS: These data show that triclosan resistance can occur via distinct pathways in Salmonella, and demonstrate a novel triclosan resistance network that is likely to have relevance to other pathogenic bacteria subject to triclosan exposure and may provide new targets for development of antimicrobial agents.
Authors: Karin D Rodland; Joshua N Adkins; Charles Ansong; Saiful Chowdhury; Nathan P Manes; Liang Shi; Hyunjin Yoon; Richard D Smith; Fred Heffron Journal: Future Microbiol Date: 2008-12 Impact factor: 3.165
Authors: Mark A Webber; Rebekah N Whitehead; Manuella Mount; Nick J Loman; Mark J Pallen; Laura J V Piddock Journal: J Antimicrob Chemother Date: 2015-05-07 Impact factor: 5.790
Authors: Mark A Webber; Vito Ricci; Rebekah Whitehead; Meha Patel; Maria Fookes; Alasdair Ivens; Laura J V Piddock Journal: mBio Date: 2013-07-23 Impact factor: 7.867
Authors: Susana Correia; Júlio D Nunes-Miranda; Luís Pinto; Hugo M Santos; María de Toro; Yolanda Sáenz; Carmen Torres; José Luis Capelo; Patrícia Poeta; Gilberto Igrejas Journal: Int J Mol Sci Date: 2014-08-15 Impact factor: 5.923