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Literature DB >> 1838699

Preparation of hydrazino-modified proteins and their use for the synthesis of 99mTc-protein conjugates.

D A Schwartz¹, M J Abrams, M M Hauser, F E Gaul, S K Larsen, D Rauh, J A Zubieta.

Abstract

The syntheses and protein linking properties of succinimidyl 4-hydrazinobenzoate hydrochloride (SHBH) and succinimidyl 6-hydrazinonicotinate hydrochloride (SHNH), two new heterobifunctional linkers which lead to hydrazino-modified proteins, are described. SHBH-modified proteins are unstable due to the presence of the phenylhydrazine moiety. This problem was overcome by synthesizing the hydrazinopyridine analogue SHNH, and the conjugates derived from this linker are stable. Tc(V) oxo precursors readily add to hydrazinopyridine-modified proteins to yield the desired 99mTc-radiolabeled protein. 99mTc-hydrazinopyridine-polyclonal IgG conjugates are useful agents for the imaging of focal sites of infection.

Entities: Chemical Disease

Mesh：

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Year: 1991 PMID： 1838699 DOI： 10.1021/bc00011a007

Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774

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Cited

12 in total

1. (99m)Tc-bioorthogonal click chemistry reagent for in vivo pretargeted imaging.

Authors: María Fernanda García; Xiuli Zhang; Manankumar Shah; Jessica Newton-Northup; Pablo Cabral; Hugo Cerecetto; Thomas Quinn
Journal: Bioorg Med Chem Date: 2016-01-29 Impact factor: 3.641

2. A novel ternary ligand system useful for preparation of cationic (99m)Tc-diazenido complexes and (99m)Tc-labeling of small biomolecules.

Authors: Young-Seung Kim; Zhengjie He; Wen-Yuan Hsieh; Shuang Liu
Journal: Bioconjug Chem Date: 2006 Mar-Apr Impact factor: 4.774

Review 3. Bifunctional coupling agents for radiolabeling of biomolecules and target-specific delivery of metallic radionuclides.

Authors: Shuang Liu
Journal: Adv Drug Deliv Rev Date: 2008-04-23 Impact factor: 15.470

Preparation of hydrazino-modified proteins and their use for the synthesis of 99mTc-protein conjugates.

1. (99m)Tc-bioorthogonal click chemistry reagent for in vivo pretargeted imaging.

2. A novel ternary ligand system useful for preparation of cationic (99m)Tc-diazenido complexes and (99m)Tc-labeling of small biomolecules.

Review 3. Bifunctional coupling agents for radiolabeling of biomolecules and target-specific delivery of metallic radionuclides.

4. Metallic radionuclides in the development of diagnostic and therapeutic radiopharmaceuticals.

5. Aromatic aldehyde and hydrazine activated peptide coated quantum dots for easy bioconjugation and live cell imaging.

6. Single-step conjugation of bioactive peptides to proteins via a self-contained succinimidyl bis-arylhydrazone.

7. Evaluation of K(HYNIC)(2) as a bifunctional chelator for (99m)Tc-labeling of small biomolecules.

8. Rapid oxime and hydrazone ligations with aromatic aldehydes for biomolecular labeling.

9. Fluorescence quenchers for hydrazone and oxime orthogonal bioconjugation.

Review 10. Radiolabeling with technetium-99m to study high-capacity and low-capacity biochemical systems.