Zhaogui Sun1, Renwei Su, Zengming Yang, Huijuan Shi, Chengquan Liu, Jian Wang. 1. National Population and Family Planning Committee Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research, Shanghai, People's Republic of China.
Abstract
OBJECTIVE: The aim of this study was to identify a novel implantation-related molecule and to examine EMO2 expression in the mouse uterus during the peri-implantation period. DESIGN: Experimental study. SETTING: Research laboratory. ANIMAL(S): Adult ICR mice aged 6-8 weeks. INTERVENTION(S): Adult female mice were mated with fertile males to achieve pregnancy. Implantation was delayed by ovariectomizing pregnant mice on day 4 and administering P during days 5-7; implantation was then initiated by administering E(2). Pseudopregnant mice were obtained by mating females with vasectomized males. MAIN OUTCOME MEASURE(S): The tissue distribution of EMO2 mRNA was detected by reverse transcriptase-polymerase chain reaction, and the uterine expression pattern of the EMO2 protein was determined by immunohistochemistry. RESULT(S): The full cDNA sequence of EMO2 was registered in GenBank (AY372183). EMO2 mRNA expression was observed in all mouse tissues tested. The expression of the EMO2 protein was predominately localized in decidual cells at the implantation site during days 5-6 of pregnancy, and its expression was induced by the active blastocyst and artificially induced decidualization. CONCLUSION(S): Our data indicate that EMO2 may play a key role in the mouse embryo implantation process.
OBJECTIVE: The aim of this study was to identify a novel implantation-related molecule and to examine EMO2 expression in the mouse uterus during the peri-implantation period. DESIGN: Experimental study. SETTING: Research laboratory. ANIMAL(S): Adult ICR mice aged 6-8 weeks. INTERVENTION(S): Adult female mice were mated with fertile males to achieve pregnancy. Implantation was delayed by ovariectomizing pregnant mice on day 4 and administering P during days 5-7; implantation was then initiated by administering E(2). Pseudopregnant mice were obtained by mating females with vasectomized males. MAIN OUTCOME MEASURE(S): The tissue distribution of EMO2 mRNA was detected by reverse transcriptase-polymerase chain reaction, and the uterine expression pattern of the EMO2 protein was determined by immunohistochemistry. RESULT(S): The full cDNA sequence of EMO2 was registered in GenBank (AY372183). EMO2 mRNA expression was observed in all mouse tissues tested. The expression of the EMO2 protein was predominately localized in decidual cells at the implantation site during days 5-6 of pregnancy, and its expression was induced by the active blastocyst and artificially induced decidualization. CONCLUSION(S): Our data indicate that EMO2 may play a key role in the mouse embryo implantation process.