BACKGROUND: MicroRNAs (miRNAs) are approximately 22 nucleotide long, non-coding RNAs that regulate gene expression by binding to the 3'-untranslated region of target mRNAs and also a variety of cellular processes. It has recently been established that dysregulation of miRNA expression can be detected in the majority of human cancers. A variety of high-throughput screening methods has been developed to identify dysregulated miRNA species in tumours. For retrospective clinical studies formalin-fixed, paraffin-embedded (FFPE) tissue is the most widely used material. MATERIALS AND METHODS: The miRNA expression profiles of freshly frozen (CRYO) and FFPE tissues of seven tonsil and four liver samples were compared, using a qPCR-based assay, profiling 157 miRNA species. RESULTS: The significance of miRNA-profiles was barely influenced by FFPE treatment in both tissues and the variance induced by FFPE treatment was much smaller than the variance caused by biologically based differential expression. CONCLUSION: FFPE material is well suited for miRNA profiling.
BACKGROUND: MicroRNAs (miRNAs) are approximately 22 nucleotide long, non-coding RNAs that regulate gene expression by binding to the 3'-untranslated region of target mRNAs and also a variety of cellular processes. It has recently been established that dysregulation of miRNA expression can be detected in the majority of humancancers. A variety of high-throughput screening methods has been developed to identify dysregulated miRNA species in tumours. For retrospective clinical studies formalin-fixed, paraffin-embedded (FFPE) tissue is the most widely used material. MATERIALS AND METHODS: The miRNA expression profiles of freshly frozen (CRYO) and FFPE tissues of seven tonsil and four liver samples were compared, using a qPCR-based assay, profiling 157 miRNA species. RESULTS: The significance of miRNA-profiles was barely influenced by FFPE treatment in both tissues and the variance induced by FFPE treatment was much smaller than the variance caused by biologically based differential expression. CONCLUSION: FFPE material is well suited for miRNA profiling.
Authors: Caroline Conway; Angana Mitra; Rosalyn Jewell; Juliette Randerson-Moor; Samira Lobo; Jérémie Nsengimana; Sara Edward; D Scott Sanders; Martin Cook; Barry Powell; Andy Boon; Faye Elliott; Floor de Kort; Margaret A Knowles; D Timothy Bishop; Julia Newton-Bishop Journal: Clin Cancer Res Date: 2009-11-03 Impact factor: 12.531
Authors: Barbara Muciaccia; Carmen Vico; Mariarosaria Aromatario; Francesco Fazi; Rossana Cecchi Journal: Int J Legal Med Date: 2014-08-19 Impact factor: 2.686
Authors: Rashmi S Goswami; Levi Waldron; Jerry Machado; Nilva K Cervigne; Wei Xu; Patricia P Reis; Denis J Bailey; Igor Jurisica; Michael R Crump; Suzanne Kamel-Reid Journal: BMC Biotechnol Date: 2010-06-23 Impact factor: 2.563