| Literature DB >> 18381079 |
Fei Yan1, Wen-Kui Yang, Xin-Yang Li, Ting-Ting Lin, Yan-Ni Lun, Feng Lin, Shao-Wu Lv, Gang-Lin Yan, Jun-Qiu Liu, Jia-Cong Shen, Ying Mu, Gui-Min Luo.
Abstract
Superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST) and glutathione reductase (GR) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS, as not one of them can singlehandedly clear all forms of ROS. In order to imitate the synergy of the enzymes, we designed and generated a recombinant protein, which comprises of a Schistosoma japonicum GST (SjGST) and a bifunctional 35-mer peptide with SOD and GPX activities. The engineered protein demonstrated SOD, GPX and GST activities simultaneously. This trifunctional enzyme with SOD, GPX and GST activities is expected to be the best ROS scavenger.Entities:
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Year: 2008 PMID： 18381079 DOI： 10.1016/j.bbagen.2008.03.003
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002