Simultaneous multicomponent analysis of overlapping spectrophotometric signals using a wavelet-based latent variable regression.

A wavelet-based latent variable regression (WLVR) method was developed to perform simultaneous quantitative analysis of overlapping spectrophotometric signals. The quality of the noise removal was improved by combining wavelet thresholding with principal component analysis (PCA). A method for selecting the optimum threshold was also developed. Eight error functions were calculated for deducing the number of factor. The latent variables were made by projecting the wavelet-processed signals onto orthogonal basis eigenvectors. Two-programs WMRA and WLVR, were designed to perform wavelet thresholding and simultaneous multicomponent determination. Experimental results showed the WLVR method to be successful even where there was severe overlap of spectra.