Literature DB >> 18377922

The biological activity of a recombinantly expressed (His)(6)-tagged peanut allergen (rAra h 1) is unaffected by endotoxin removal.

Louise Bjerremann Jensen1, Anna Maria Torp, Sven Bode Andersen, Per Stahl Skov, Lars K Poulsen, Edward F Knol, Els van Hoffen.   

Abstract

The application of recombinant (His)(6)-tagged proteins in cell culture assays is associated with problems due to lipopolysaccharide (LPS) contamination. LPS stimulates cells of the immune system, thereby masking antigen-specific activation of T cells. Due to the affinity of LPS for histidine it is associated with difficulties to remove LPS from recombinant (His)(6)-tagged proteins. Here we describe that the Triton X-114 phase separation method can be used to remove LPS from (His)(6)-tagged proteins and that the recombinant proteins retain their biological activity.

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Year:  2008        PMID： 18377922     DOI： 10.1016/j.jim.2008.02.012

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  10 in total

1.  Adenylate kinase: a novel antigen for immunodiagnosis and subunit vaccine against tuberculosis.

Authors:  Yangjiong Xiao; Wei Sha; Zhaofeng Tian; Yingying Chen; Ping Ji; Qin Sun; Huiyu Wang; Shujun Wang; Yong Fang; Han-Li Wen; Hui-Min Zhao; Jie Lu; Heping Xiao; Xiao-Yong Fan; Hao Shen; Ying Wang
Journal:  J Mol Med (Berl)       Date:  2016-02-23       Impact factor: 4.599

2.  The protease allergen Pen c 13 induces allergic airway inflammation and changes in epithelial barrier integrity and function in a murine model.

Authors:  Jui-Chieh Chen; Jiing-Guang Chuang; Yu-Yi Su; Bor-Luen Chiang; You-Shuei Lin; Lu-Ping Chow
Journal:  J Biol Chem       Date:  2011-05-25       Impact factor: 5.157

3.  Protein polymer hydrogels: effects of endotoxin on biocompatibility.

Authors:  Liese N Beenken-Rothkopf; Lindsay S Karfeld-Sulzer; Xiaomin Zhang; Hermann Kissler; Sara A Michie; Dixon B Kaufman; Magali J Fontaine; Annelise E Barron
Journal:  J Biomater Appl       Date:  2012-07-24       Impact factor: 2.712

4.  A recombinant chimeric protein specifically induces mutant KRAS degradation and potently inhibits pancreatic tumor growth.

Authors:  Ting Pan; Yiwen Zhang; Nan Zhou; Xin He; Cancan Chen; Liting Liang; Xiaobing Duan; Yingtong Lin; Kang Wu; Hui Zhang
Journal:  Oncotarget       Date:  2016-07-12

5.  Determination of Lipoprotein Z-Specific IgA in Tuberculosis and Latent Tuberculosis Infection.

Authors:  Jia-Ni Xiao; Yanqing Xiong; Yingying Chen; Yang-Jiong Xiao; Ping Ji; Yong Li; Shu-Jun Wang; Guo-Ping Zhao; Qi-Jian Cheng; Shui-Hua Lu; Ying Wang
Journal:  Front Cell Infect Microbiol       Date:  2017-11-30       Impact factor: 5.293

6.  Optimized Triton X-114 assisted lipopolysaccharide (LPS) removal method reveals the immunomodulatory effect of food proteins.

Authors:  Malgorzata Teodorowicz; Olaf Perdijk; Iris Verhoek; Coen Govers; Huub F J Savelkoul; Yongfu Tang; Harry Wichers; Kerensa Broersen
Journal:  PLoS One       Date:  2017-03-29       Impact factor: 3.240

7.  Biological Activity of Masked Endotoxin.

Authors:  Harald Schwarz; Jan Gornicec; Theresa Neuper; Maria Alejandra Parigiani; Michael Wallner; Albert Duschl; Jutta Horejs-Hoeck
Journal:  Sci Rep       Date:  2017-03-20       Impact factor: 4.379

8.  Secretory expression and purification of recombinant Escherichia coli heat-labile enterotoxin B subunit and its applications on intranasal vaccination of hantavirus.

Authors:  Shouchun Cao; Ying Zhang; Feng Liu; Qin Wang; Quanfu Zhang; Qinzhi Liu; Chuan Li; Mifang Liang; Dexin Li
Journal:  Mol Biotechnol       Date:  2008-12-17       Impact factor: 2.695

9.  Schistosoma japonicum egg specific protein SjE16.7 recruits neutrophils and induces inflammatory hepatic granuloma initiation.

Authors:  Chenyun Wu; Qing Chen; Yan Fang; Jianhua Wu; Yanyan Han; Ying Wang; Yang Yang; Min Chu; Yan Feng; Linping Tan; Xiaokui Guo; Wei Hu; Zhaojun Wang
Journal:  PLoS Negl Trop Dis       Date:  2014-02-13

10.  Differences in the intrinsic immunogenicity and allergenicity of Bet v 1 and related food allergens revealed by site-directed mutagenesis.

Authors:  A Roulias; U Pichler; M Hauser; M Himly; H Hofer; P Lackner; C Ebner; P Briza; B Bohle; M Egger; M Wallner; F Ferreira
Journal:  Allergy       Date:  2013-11-14       Impact factor: 13.146
  10 in total

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