OBJECTIVES: Interleukin (IL) 22 is a recently identified T-cell-derived cytokine. IL-22 binds at the cell surface to a heterodimer receptor complex composed of IL-22 receptor (R) 1 and IL-10R2. In this study, we performed immunohistochemical analyses for IL-22R1 expression in human pancreatic tissue. METHODS: Normal human pancreatic tissue (n = 8) was immunostained with antihuman IL-22R1 antibodies following standard immunohistochemical procedures. RESULTS: In the normal human pancreas, IL-22R1 was expressed in the islets of Langerhans. IL-22R1 was not expressed by the acinar cells and ductal epithelium. Double-immunostaining experiments showed that the majority of insulin-expressing beta cells and glucagon-expressing alpha cells were immunopositive for IL-22R1. CONCLUSIONS: The islets of Langerhans are the local site for IL-22R1 expression in the human pancreas. It may be that the T-cell-mediated immune response modulates cell islet function through IL-22 signaling.
OBJECTIVES:Interleukin (IL) 22 is a recently identified T-cell-derived cytokine. IL-22 binds at the cell surface to a heterodimer receptor complex composed of IL-22 receptor (R) 1 and IL-10R2. In this study, we performed immunohistochemical analyses for IL-22R1 expression in humanpancreatic tissue. METHODS: Normal humanpancreatic tissue (n = 8) was immunostained with antihuman IL-22R1 antibodies following standard immunohistochemical procedures. RESULTS: In the normal human pancreas, IL-22R1 was expressed in the islets of Langerhans. IL-22R1 was not expressed by the acinar cells and ductal epithelium. Double-immunostaining experiments showed that the majority of insulin-expressing beta cells and glucagon-expressing alpha cells were immunopositive for IL-22R1. CONCLUSIONS: The islets of Langerhans are the local site for IL-22R1 expression in the human pancreas. It may be that the T-cell-mediated immune response modulates cell islet function through IL-22 signaling.
Authors: Sumaira Z Hasnain; Danielle J Borg; Brooke E Harcourt; Hui Tong; Yonghua H Sheng; Choa Ping Ng; Indrajit Das; Ran Wang; Alice C-H Chen; Thomas Loudovaris; Thomas W Kay; Helen E Thomas; Jonathan P Whitehead; Josephine M Forbes; Johannes B Prins; Michael A McGuckin Journal: Nat Med Date: 2014-11-02 Impact factor: 53.440