BACKGROUND: One of the major consequences in beta- thalassemia is iron overload. Oxidative statuses have been reported in beta-thalassemia patients by several studies. It has been proven that iron plays a critical role in the formation of reactive oxygen species (ROS). More recently, we have found the induction of Lcn2/NGAL expression under oxidative stress condition. In this study, it was assumed that NGAL should be upregulated in beta-thalassemia patients because of oxidative stress condition. METHODS: Assessment of NGAL expressions in 25 adult beta-thalassemia and 9 pediatric patients was performed by semiquantitative RT-PCR, real-time RT-PCR and ELISA. RESULTS: Adult beta-thalassemia patients upregulated NGAL expression compared with the normal samples but no upregulation was observed in pediatric patients. CONCLUSIONS: Upregulation may play an important role in decreasing ROS or iron in beta-thalassemia patients.
BACKGROUND: One of the major consequences in beta- thalassemia is iron overload. Oxidative statuses have been reported in beta-thalassemiapatients by several studies. It has been proven that iron plays a critical role in the formation of reactive oxygen species (ROS). More recently, we have found the induction of Lcn2/NGAL expression under oxidative stress condition. In this study, it was assumed that NGAL should be upregulated in beta-thalassemiapatients because of oxidative stress condition. METHODS: Assessment of NGAL expressions in 25 adult beta-thalassemia and 9 pediatric patients was performed by semiquantitative RT-PCR, real-time RT-PCR and ELISA. RESULTS: Adult beta-thalassemiapatients upregulated NGAL expression compared with the normal samples but no upregulation was observed in pediatric patients. CONCLUSIONS: Upregulation may play an important role in decreasing ROS or iron in beta-thalassemiapatients.
Authors: A M F Martines; R Masereeuw; H Tjalsma; J G Hoenderop; J F M Wetzels; D W Swinkels Journal: Nat Rev Nephrol Date: 2013-05-14 Impact factor: 28.314
Authors: Adrian Mellor; Christopher Boos; Mike Stacey; Tim Hooper; Chris Smith; Joe Begley; Jo Yarker; Rick Piper; John O'Hara; Rod King; Steve Turner; David R Woods Journal: Dis Markers Date: 2013-10-20 Impact factor: 3.434