| Literature DB >> 18375163 |
Mitsuhiro Ueda1, Tomohiko Asano, Masami Nakazawa, Kazutaka Miyatake, Kuniyo Inouye.
Abstract
Novel raw-starch-digesting and cold-adapted alpha-amylases (Amy I and Amy II) from the earthworm Eisenia foetida were purified to electrophoretically homogeneous states. The molecular weights of both purified enzymes were estimated to be 60,000 by SDS-PAGE. The enzymes were most active at pH 5.5 and 50 degrees C and stable at pH 7.0-9.0 and 50-60 degrees C. Both Amy I and II exhibited activities at 10 degrees C. The enzymes were inhibited by metal ions Cu(2+), Fe(2+), and Hg(2+), and hydrolyzed raw starch into glucose, maltose and maltotriose as end products.Entities:
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Year: 2008 PMID： 18375163 DOI： 10.1016/j.cbpb.2008.02.003
Source DB: PubMed Journal: Comp Biochem Physiol B Biochem Mol Biol ISSN： 1096-4959 Impact factor: 2.231