OBJECTIVE: The aim of this study was to investigate whether genotypes of Streptococcus mutans strain can be detected as effectively in saliva samples as in plaque samples from buccal surfaces and occlusal surface fissures of permanent first molars. DESIGN: The study included 20 school children aged 6-7 years who were positive for mutans streptococci. Samples of stimulated saliva and of dental plaque on buccal surfaces and occlusal surface fissures of permanent first molars were collected. Samples were cultivated in MSB agar. Up to nine isolates compatible with mutans streptococci were obtained and identified by means of biochemical tests. All isolates identified as S. mutans were genotyped by arbitrarily primed polymerase chain reaction. RESULTS: A total of 28 genotypes of S. mutans were isolated: 23 in saliva samples, 23 in buccal surface plaque samples, and 16 in plaque samples from occlusal surface fissures. CONCLUSIONS: Although, saliva sampling did not reveal all genotypes isolated, it was equally as effective as plaque sampling from the buccal surfaces of permanent first molars, and more effective than plaque sampling from fissures on their occlusal surfaces.
OBJECTIVE: The aim of this study was to investigate whether genotypes of Streptococcus mutans strain can be detected as effectively in saliva samples as in plaque samples from buccal surfaces and occlusal surface fissures of permanent first molars. DESIGN: The study included 20 school children aged 6-7 years who were positive for mutans streptococci. Samples of stimulated saliva and of dental plaque on buccal surfaces and occlusal surface fissures of permanent first molars were collected. Samples were cultivated in MSB agar. Up to nine isolates compatible with mutans streptococci were obtained and identified by means of biochemical tests. All isolates identified as S. mutans were genotyped by arbitrarily primed polymerase chain reaction. RESULTS: A total of 28 genotypes of S. mutans were isolated: 23 in saliva samples, 23 in buccal surface plaque samples, and 16 in plaque samples from occlusal surface fissures. CONCLUSIONS: Although, saliva sampling did not reveal all genotypes isolated, it was equally as effective as plaque sampling from the buccal surfaces of permanent first molars, and more effective than plaque sampling from fissures on their occlusal surfaces.
Authors: Elizabeth A Palmer; Truman Nielsen; Patricia Peirano; Anna T Nguyen; Alex Vo; Aivan Nguyen; Stephen Jackson; Tyler Finlayson; Rebecca Sauerwein; Katie Marsh; Issac Edwards; Beth Wilmot; John Engle; John Peterson; Tom Maier; Curtis A Machida Journal: Pediatr Dent Date: 2012 Mar-Apr Impact factor: 1.874
Authors: Elizabeth A Palmer; Alex Vo; Shelby B Hiles; Patricia Peirano; Samyia Chaudhry; Amy Trevor; Iraj Kasimi; Jill Pollard; Christopher Kyles; Michael Leo; Beth Wilmot; John Engle; John Peterson; Tom Maier; Curtis A Machida Journal: J Oral Microbiol Date: 2012-12-14 Impact factor: 5.474
Authors: Alissa L Villhauer; David J Lynch; John J Warren; Deborah V Dawson; Derek R Blanchette; David R Drake Journal: Clin Exp Dent Res Date: 2017-12-22
Authors: Matilda Handsley-Davis; Emily Skelly; Newell W Johnson; Kostas Kapellas; Ratilal Lalloo; Jeroen Kroon; Laura S Weyrich Journal: Front Oral Health Date: 2021-03-18