BACKGROUND: Tumor necrosis factor (TNF)-alpha is a cytokine that initiates liver regeneration after hepatectomy (HTx), although extensive HTx can cause liver failure with significant rise in serum TNF-alpha levels. To test our hypothesis that modulation of endogenous TNF-alpha attenuates liver failure even after extensive HTx, we used ONO-SM362, a novel TNF-alpha inhibitor, in mice subjected to 85% HTx. METHODS: ICR mice were divided into 5 groups: 70% HTx, 85% HTx, 85% HTx plus ONO-SM362, 85% HTx plus monoclonal TNF-alpha antibody (mAb), and 85% HTx plus FR167653, a TNF-alpha inhibitor. We analyzed the survival rate, blood ammonia (NH(3)), serum TNF-alpha levels, TNF-alpha mRNA expression in the liver and spleen by real-time polymerase chain reaction, histologic changes, polymorphonuclear neutrophils (PMNs) infiltration, and proliferating cell nuclear antigen labeling index (PCNA LI) in the 5 groups. RESULTS: The survival rate at 7 days after surgery was 100%, 0%, 100%, 50%, and 0%, for the 70% HTx, 85% HTx, 85% HTx + ONO-SM362, 85% HTx + mAb, and 85% HTx + FR167653, respectively. Mice that underwent 85% HTx died from liver failure associated with a significant rise in serum TNF-alpha level. ONO-SM362 and mAb improved animal survival and enhanced PCNA LI. In addition, ONO-SM362 inhibited TNF-alpha mRNA expression in the remnant liver and suppressed PMNs infiltration. CONCLUSIONS: Suppression of excessive TNF-alpha production using ONO-SM362 ameliorated liver failure after 85% HTx.
BACKGROUND:Tumor necrosis factor (TNF)-alpha is a cytokine that initiates liver regeneration after hepatectomy (HTx), although extensive HTx can cause liver failure with significant rise in serum TNF-alpha levels. To test our hypothesis that modulation of endogenous TNF-alphaattenuates liver failure even after extensive HTx, we used ONO-SM362, a novel TNF-alpha inhibitor, in mice subjected to 85% HTx. METHODS: ICR mice were divided into 5 groups: 70% HTx, 85% HTx, 85% HTx plus ONO-SM362, 85% HTx plus monoclonal TNF-alpha antibody (mAb), and 85% HTx plus FR167653, a TNF-alpha inhibitor. We analyzed the survival rate, blood ammonia (NH(3)), serum TNF-alpha levels, TNF-alpha mRNA expression in the liver and spleen by real-time polymerase chain reaction, histologic changes, polymorphonuclear neutrophils (PMNs) infiltration, and proliferating cell nuclear antigen labeling index (PCNA LI) in the 5 groups. RESULTS: The survival rate at 7 days after surgery was 100%, 0%, 100%, 50%, and 0%, for the 70% HTx, 85% HTx, 85% HTx + ONO-SM362, 85% HTx + mAb, and 85% HTx + FR167653, respectively. Mice that underwent 85% HTx died from liver failure associated with a significant rise in serum TNF-alpha level. ONO-SM362 and mAb improved animal survival and enhanced PCNA LI. In addition, ONO-SM362 inhibited TNF-alpha mRNA expression in the remnant liver and suppressed PMNs infiltration. CONCLUSIONS: Suppression of excessive TNF-alpha production using ONO-SM362 ameliorated liver failure after 85% HTx.
Authors: Sebastián Katz; Orna Ernst; Dorit Avni; Muhammad Athamna; Amir Philosoph; Lide Arana; Alberto Ouro; L Alexis Hoeferlin; Michael M Meijler; Charles E Chalfant; Antonio Gómez-Muñoz; Tsaffrir Zor Journal: Immunol Lett Date: 2015-12-03 Impact factor: 3.685
Authors: Nadia F Lamour; Dayanjan S Wijesinghe; Jennifer A Mietla; Katherine E Ward; Robert V Stahelin; Charles E Chalfant Journal: J Biol Chem Date: 2011-10-18 Impact factor: 5.157
Authors: Joshua Hefler; Braulio A Marfil-Garza; Rena L Pawlick; Darren H Freed; Constantine J Karvellas; David L Bigam; A M James Shapiro Journal: PeerJ Date: 2021-12-09 Impact factor: 2.984
Authors: Katja Klemm; Christian Eipel; Daniel Cantré; Kerstin Abshagen; Michael D Menger; Brigitte Vollmar Journal: PLoS One Date: 2008-12-11 Impact factor: 3.240