Ruth Bunker Lathi1, Barry Behr. 1. Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology and Infertility, Stanford University Medical Center, Stanford, California 94305, USA. rlathi@stanford.edu
Abstract
OBJECTIVE: To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst. DESIGN: Techniques and instrumentation. SETTING: A University Medical Center. PATIENT(S): Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program. INTERVENTION(S): Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance. MAIN OUTCOME MEASUREMENT(S): Serum betahCG and transvaginal ultrasound. RESULT(S): Positive betahCG and ongoing pregnancy. CONCLUSION(S): Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.
OBJECTIVE: To report a case of a successful pregnancy after trophectoderm biopsy and three-probe fluorescent in situ hybridization of a frozen blastocyst. DESIGN: Techniques and instrumentation. SETTING: A University Medical Center. PATIENT(S): Infertility patient desiring trophectoderm biopsy on frozen blastocyst for preimplantation testing, from an IVF cycle at a referring IVF program. INTERVENTION(S): Frozen blastocysts were thawed the evening before the planned transfer. Trophectoderm biopsy was performed in the morning. The fluorescent in situ hybridization results were obtained the same day; embryo transfer was performed under ultrasound guidance. MAIN OUTCOME MEASUREMENT(S): Serum betahCG and transvaginal ultrasound. RESULT(S): Positive betahCG and ongoing pregnancy. CONCLUSION(S): Trophectoderm biopsy can be used as a means for testing frozen blastocysts in patients with excess embryos cryopreserved on day 5 or 6 from previously preformed IVF cycles.