F Cardona1, I Tunez, I Tasset, M Murri, F J Tinahones. 1. Servicio de Endocrinología y Nutrición, Hospital Clínico Universitario Virgen de Victoria, 29009 Malaga and CIBER Fisiopatología Obesidad y Nutrición (CB06/03) Instituto de Salud Carlos III, Spain.
Abstract
OBJECTIVE: We compared the levels of biomarkers of oxidative stress before and after a fat overload in three groups. MATERIALS AND METHODS: 17 controls and two groups with hypertriglyceridemia: 43 without the metabolic syndrome (TG-non-MS) and 29 with the metabolic syndrome (TG-MS). All subjects underwent a 60 g fat overload. Baseline measurements included glucose, BMI (body mass index), waist circumference and HOMA IR (homeostasis model assessment insulin resistance). Cholesterol, triglycerides, HDL (high density lipoprotein) cholesterol, TNF-alpha (tumor necrosis factor) and IL-6 (interleukin-6), lipoperoxide (LPO), carbonylated proteins, reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GSH-PX), glutathione reductase (GSH-Rd), catalase and glutathione transferase (GST) were measured at baseline and 3 h after fat overload. RESULTS: Compared to the controls, the two patient groups had higher plasma levels at baseline and after overload of cholesterol, triglycerides and apolipoprotein B, LPO, carbonylated proteins and GSSG, and lower levels of antioxidants at baseline and after the fat overload. CONCLUSION: The two patient groups had the same degree of oxidative stress.
OBJECTIVE: We compared the levels of biomarkers of oxidative stress before and after a fat overload in three groups. MATERIALS AND METHODS: 17 controls and two groups with hypertriglyceridemia: 43 without the metabolic syndrome (TG-non-MS) and 29 with the metabolic syndrome (TG-MS). All subjects underwent a 60 g fat overload. Baseline measurements included glucose, BMI (body mass index), waist circumference and HOMA IR (homeostasis model assessment insulin resistance). Cholesterol, triglycerides, HDL (high density lipoprotein) cholesterol, TNF-alpha (tumor necrosis factor) and IL-6 (interleukin-6), lipoperoxide (LPO), carbonylated proteins, reduced glutathione (GSH), oxidized glutathione (GSSG), glutathione peroxidase (GSH-PX), glutathione reductase (GSH-Rd), catalase and glutathione transferase (GST) were measured at baseline and 3 h after fat overload. RESULTS: Compared to the controls, the two patient groups had higher plasma levels at baseline and after overload of cholesterol, triglycerides and apolipoprotein B, LPO, carbonylated proteins and GSSG, and lower levels of antioxidants at baseline and after the fat overload. CONCLUSION: The two patient groups had the same degree of oxidative stress.
Authors: Mora Murri; Eduardo García-Fuentes; José Manuel García-Almeida; Lourdes Garrido-Sánchez; María Dolores Mayas; Rosa Bernal; Francisco J Tinahones Journal: Obes Surg Date: 2010-03 Impact factor: 4.129
Authors: M Clemente-Postigo; M I Queipo-Ortuño; M Murri; M Boto-Ordoñez; P Perez-Martinez; C Andres-Lacueva; F Cardona; F J Tinahones Journal: J Lipid Res Date: 2012-03-05 Impact factor: 5.922
Authors: Katarzyna Zabłocka-Słowińska; Sylwia Płaczkowska; Katarzyna Skórska; Anna Prescha; Konrad Pawełczyk; Irena Porębska; Monika Kosacka; Halina Grajeta Journal: PLoS One Date: 2019-04-11 Impact factor: 3.240