Literature DB >> 18370282

Geminivirus: biolistic inoculation and molecular diagnosis.

Anésia A Santos1, Lilian H Florentino, Acássia B L Pires, Elizabeth P B Fontes.   

Abstract

The Geminiviridae family is a large family of plant viruses that has single-stranded DNA genomes and infects a large variety of crop species. In this chapter, we describe a biolistic inoculation protocol that has been successfully used to propagate new species of geminivirus in permissive hosts with total DNA extracted from infected plants. This allows us to directly investigate the biological properties of uncloned and not sap-transmissible geminiviruses.

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Year:  2008        PMID： 18370282     DOI： 10.1007/978-1-59745-102-4_39

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  5 in total

1.  Functional analysis of a novel motif conserved across geminivirus Rep proteins.

Authors:  Tara E Nash; Mary B Dallas; Maria Ines Reyes; Gregory K Buhrman; J Trinidad Ascencio-Ibañez; Linda Hanley-Bowdoin
Journal:  J Virol       Date:  2010-11-17       Impact factor: 5.103

2.  NIK1-mediated translation suppression functions as a plant antiviral immunity mechanism.

Authors:  Cristiane Zorzatto; João Paulo B Machado; Kênia V G Lopes; Kelly J T Nascimento; Welison A Pereira; Otávio J B Brustolini; Pedro A B Reis; Iara P Calil; Michihito Deguchi; Gilberto Sachetto-Martins; Bianca C Gouveia; Virgílio A P Loriato; Marcos A C Silva; Fabyano F Silva; Anésia A Santos; Joanne Chory; Elizabeth P B Fontes
Journal:  Nature       Date:  2015-02-23       Impact factor: 49.962

3.  Conserved threonine residues within the A-loop of the receptor NIK differentially regulate the kinase function required for antiviral signaling.

Authors:  Anésia A Santos; Claudine M Carvalho; Lilian H Florentino; Humberto J O Ramos; Elizabeth P B Fontes
Journal:  PLoS One       Date:  2009-06-03       Impact factor: 3.240

4.  Regulated nuclear trafficking of rpL10A mediated by NIK1 represents a defense strategy of plant cells against virus.

Authors:  Claudine M Carvalho; Anésia A Santos; Silvana R Pires; Carolina S Rocha; Daniela I Saraiva; João Paulo B Machado; Eliciane C Mattos; Luciano G Fietto; Elizabeth P B Fontes
Journal:  PLoS Pathog       Date:  2008-12-26       Impact factor: 6.823

5.  A VIGS screen identifies immunity in the Arabidopsis Pla-1 accession to viruses in two different genera of the Geminiviridae.

Authors:  Maria Ines Reyes; Miguel A Flores-Vergara; Orlene Guerra-Peraza; Cyprian Rajabu; Jigar Desai; Yokiko H Hiromoto-Ruiz; Joseph Ndunguru; Linda Hanley-Bowdoin; Susanne Kjemtrup; Jose T Ascencio-Ibáñez; Dominique Robertson
Journal:  Plant J       Date:  2017-10-24       Impact factor: 6.417
  5 in total

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