| Literature DB >> 18369785 |
Wenyi Gu1, Lisa Putral, Nigel McMillan.
Abstract
We describe the protocols of using siRNAs, or shRNAs delivered by a lentiviral vector, as a means to silence cancer-causing genes. We use cervical cancer as a model to demonstrate the inhibition of the human papillomavirus (HPV) oncogenes E6 and E7 in cervical cancer cells by RNAi and inhibition of the cell growth in vitro and tumor growth in mouse models. The protocols include methods on siRNA and shRNA design, production of lentiviral-vectored shRNA, transfection or transduction of cervical cancer cells with siRNA or shRNA, and detection of the inhibitory effects of siRNA or shRNA both in vitro and in vitro.Entities:
Mesh：
Substances：
Year: 2008 PMID： 18369785 DOI： 10.1007/978-1-59745-191-8_12
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745