| Literature DB >> 18369774 |
Mohammed Amarzguioui1, John J Rossi.
Abstract
An efficient RNAi largely depends on optimal design of the siRNA. In recent studies, Dicer substrates were found to be more potent than classical synthetic 21-mer siRNAs, suggesting a coupling of the Dicer-mediated processing step to the efficient assembly of the silencing complex, RISC. We describe the fundamental principles and experimental results leading to optimal Dicer substrates.Entities:
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Year: 2008 PMID： 18369774 DOI： 10.1007/978-1-59745-191-8_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745