OBJECTIVE: To analyze the effects of GnRH agonists versus antagonists on mitochondrial distribution and activity in human mature oocytes. DESIGN: Randomized research experimental study. SETTING: Academic basic research laboratory and hospital-based fertility center. PATIENT(S): Two hundred twenty-five supernumerary mature oocytes from 44 patients. INTERVENTION(S): Fluorescent staining and confocal laser scanning microscopy on oocytes after the use of either GnRH agonist (group A) or GnRH antagonist (group B). MAIN OUTCOME MEASURE(S): Oocyte mitochondrial distribution pattern and activity using MitoTracker Orange CMTM Ros. RESULT(S): More oocytes showing polarized mitochondrial distribution pattern were found in group A than in group B (35% vs. 14%). In group B, hCG rather than GnRH agonist, for ovulation induction, resulted in more oocytes showing heterogeneous (57% vs. 14%), in particular polarized (24% vs. 0) mitochondrial distribution. In groups A and B, fluorescence intensity did not vary according to mitochondrial distribution pattern. However, fluorescence intensity was higher in oocytes with polarized and large granules configurations in group B compared to group A. CONCLUSION(S): The GnRH agonist and antagonist may have different effects on oocyte mitochondrial distribution pattern and activity. The GnRH antagonist may induce mitochondrial hyperactivity, which may be detrimental to the oocyte.
RCT Entities:
OBJECTIVE: To analyze the effects of GnRH agonists versus antagonists on mitochondrial distribution and activity in human mature oocytes. DESIGN: Randomized research experimental study. SETTING: Academic basic research laboratory and hospital-based fertility center. PATIENT(S): Two hundred twenty-five supernumerary mature oocytes from 44 patients. INTERVENTION(S): Fluorescent staining and confocal laser scanning microscopy on oocytes after the use of either GnRH agonist (group A) or GnRH antagonist (group B). MAIN OUTCOME MEASURE(S): Oocyte mitochondrial distribution pattern and activity using MitoTracker Orange CMTM Ros. RESULT(S): More oocytes showing polarized mitochondrial distribution pattern were found in group A than in group B (35% vs. 14%). In group B, hCG rather than GnRH agonist, for ovulation induction, resulted in more oocytes showing heterogeneous (57% vs. 14%), in particular polarized (24% vs. 0) mitochondrial distribution. In groups A and B, fluorescence intensity did not vary according to mitochondrial distribution pattern. However, fluorescence intensity was higher in oocytes with polarized and large granules configurations in group B compared to group A. CONCLUSION(S): The GnRH agonist and antagonist may have different effects on oocyte mitochondrial distribution pattern and activity. The GnRH antagonist may induce mitochondrial hyperactivity, which may be detrimental to the oocyte.
Authors: M E Dell'Aquila; L Bogliolo; R Russo; N A Martino; M Filioli Uranio; F Ariu; F Amati; A M Sardanelli; V Linsalata; M G Ferruzzi; A Cardinali; F Minervini Journal: Biomed Res Int Date: 2014-02-25 Impact factor: 3.411
Authors: Monzó Ana; Montañana Vicente; Rubio José María; García-Gimeno Trinidad; Romeu Alberto Journal: Clin Med Insights Reprod Health Date: 2011-02-22
Authors: Keun Jung Kim; Ju Lan Chun; Kyung-Bon Lee; Ji Hye Lee; Kang-Sun Park; Kil Woo Han; Bo Myeong Lee; Eun Young Kim; Jin Man Kim; Min Kyu Kim Journal: J Assist Reprod Genet Date: 2016-05-17 Impact factor: 3.357