Literature DB >> 18364793

Characterizing Mammalian cells and cell phantoms by polarized backscattering fiber-optic measurements.

J R Mourant, T M Johnson, J P Freyer.   

Abstract

Fiber-optic, polarized elastic-scattering spectroscopy techniques are implemented and demonstrated as a method for determining both scatterer size and concentration in highly scattering media. Measurements of polystyrene spheres are presented to validate the technique. Measurements of biological cells provide an estimate of the average effective scatterer radius of 0.5-1.0 mum. This average effective scatterer size is significantly smaller than the nucleus. In addition, to facilitate use of polarization techniques on biological cells, polarized angular dependent scattering from cell suspensions was measured. The light scattering from cells has properties similar to those of small spheres.

Entities:  

Year:  2001        PMID： 18364793     DOI： 10.1364/ao.40.005114

Source DB:  PubMed          Journal:  Appl Opt        ISSN： 1559-128X            Impact factor:   1.980


  15 in total

1.  Differences in forward angular light scattering distributions between M1 and M2 macrophages.

Authors:  David L Halaney; Aydin Zahedivash; Jennifer E Phipps; Tianyi Wang; Jordan Dwelle; Claude Jourdan Le Saux; Reto Asmis; Thomas E Milner; Marc D Feldman
Journal:  J Biomed Opt       Date:  2015-11       Impact factor: 3.170

2.  Light scattering from intact cells reports oxidative-stress-induced mitochondrial swelling.

Authors:  Jeremy D Wilson; Chad E Bigelow; David J Calkins; Thomas H Foster
Journal:  Biophys J       Date:  2005-01-14       Impact factor: 4.033

3.  Noninvasive in vivo tomographic optical imaging of cellular morphology in the breast: possible convergence of microscopic pathology and macroscopic radiology.

Authors:  Changqing Li; Stephen R Grobmyer; Nicole Massol; Xiaoping Liang; Qizhi Zhang; Lin Chen; Laurie L Fajardo; Huabei Jiang
Journal:  Med Phys       Date:  2008-06       Impact factor: 4.071

4.  Perturbation Monte Carlo methods for tissue structure alterations.

Authors:  Jennifer Nguyen; Carole K Hayakawa; Judith R Mourant; Jerome Spanier
Journal:  Biomed Opt Express       Date:  2013-09-04       Impact factor: 3.732

5.  Spectroscopic sensitive polarimeter for biomedical applications.

Authors:  Jessica C Ramella-Roman; Amritha Nayak; Scott A Prahl
Journal:  J Biomed Opt       Date:  2011-04       Impact factor: 3.170

6.  Polarized light imaging specifies the anisotropy of light scattering in the superficial layer of a tissue.

Authors:  Steven L Jacques; Stéphane Roussel; Ravikant Samatham
Journal:  J Biomed Opt       Date:  2016-07-01       Impact factor: 3.170

7.  Detection of cervical intraepithelial neoplasias and cancers in cervical tissue by in vivo light scattering.

Authors:  Judith R Mourant; Thérese J Bocklage; Tamara M Powers; Heather M Greene; Maxine H Dorin; Alan G Waxman; Meggan M Zsemlye; Harriet O Smith
Journal:  J Low Genit Tract Dis       Date:  2009-10       Impact factor: 1.925

8.  Application of Mie theory to assess structure of spheroidal scattering in backscattering geometries.

Authors:  Kevin J Chalut; Michael G Giacomelli; Adam Wax
Journal:  J Opt Soc Am A Opt Image Sci Vis       Date:  2008-08       Impact factor: 2.129

9.  Phase contrast microscopy analysis of breast tissue: differences in benign vs. malignant epithelium and stroma.

Authors:  Wendy A Wells; Xin Wang; Charles P Daghlian; Keith D Paulsen; Brian W Pogue
Journal:  Anal Quant Cytol Histol       Date:  2009-08       Impact factor: 0.302

10.  In vivo light scattering for the detection of cancerous and precancerous lesions of the cervix.

Authors:  Judith R Mourant; Tamara M Powers; Thérese J Bocklage; Heather M Greene; Maxine H Dorin; Alan G Waxman; Meggan M Zsemlye; Harriet O Smith
Journal:  Appl Opt       Date:  2009-04-01       Impact factor: 1.980
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