OBJECTIVE: Cell cycle arrest by FOXO transcription factors involves in transcriptional activation of p27(kip1), although the exact mechanism remains unclear. And it has been evidenced that reduced level of p27(kip1) which is frequently occurred in human cancers has been associated with poor prognosis. In this study, our purpose is to investigate the clinical relevance of altered patterns of Foxo3a and p27(kip1) expression in Chinese patients with localized non-Hodgkin's lymphomas (NHL). METHODS: We analyzed the Foxo3a and p27(kip1) expression of Chinese NHL patients by immunohistochemistry and protein levels using Western Blot. RESULTS: There was a direct relationship between the low level of Foxo3a and the rapid proliferation in immunohistochemical analyses. We also found a positive correlation between Foxo3a and p27(kip1) in immunohistochemical analyses and cell culture. Additionally we revealed that activation of Foxo3a could induce the accumulation of p27(kip1) at protein levels when cell cycle was arrested. CONCLUSIONS: The expression of Foxo3a may be correlated with patients' survival.
OBJECTIVE: Cell cycle arrest by FOXO transcription factors involves in transcriptional activation of p27(kip1), although the exact mechanism remains unclear. And it has been evidenced that reduced level of p27(kip1) which is frequently occurred in humancancers has been associated with poor prognosis. In this study, our purpose is to investigate the clinical relevance of altered patterns of Foxo3a and p27(kip1) expression in Chinese patients with localized non-Hodgkin's lymphomas (NHL). METHODS: We analyzed the Foxo3a and p27(kip1) expression of Chinese NHLpatients by immunohistochemistry and protein levels using Western Blot. RESULTS: There was a direct relationship between the low level of Foxo3a and the rapid proliferation in immunohistochemical analyses. We also found a positive correlation between Foxo3a and p27(kip1) in immunohistochemical analyses and cell culture. Additionally we revealed that activation of Foxo3a could induce the accumulation of p27(kip1) at protein levels when cell cycle was arrested. CONCLUSIONS: The expression of Foxo3a may be correlated with patients' survival.
Authors: Steven M Kornblau; Neera Singh; YiHua Qiu; Wenjing Chen; Nianxiang Zhang; Kevin R Coombes Journal: Clin Cancer Res Date: 2010-03-09 Impact factor: 12.531
Authors: Amparo Perez; Ana Loizaga; Raquel Arceo; Isabel Lacasa; Ainara Rabade; Kerman Zorroza; David Mosen-Ansorena; Esperanza Gonzalez; Ana M Aransay; Juan M Falcon-Perez; Miguel Unda-Urzaiz; Felix Royo Journal: Cancers (Basel) Date: 2014-01-22 Impact factor: 6.639