AIMS: In this study, we have evaluated the impact of methodological approaches in the determination of biofilm formation by four clinical isolates of Escherichia coli in static assays. METHODS AND RESULTS: The assays were performed in microtitre plates with two minimal and two enriched broths, with one- or two-steps protocol, and using three different mathematical formulas to quantify adherent bacteria. Different biofilm formation patterns were found depending on the E. coli strain, culture medium and reading optical density on one- and two-steps protocol. Strong or moderate biofilm formation occurred mostly in minimal media. The mathematical formulas used to quantify biofilm formation also gave different results and bacterial growth rate should be taken into account to quantify biofilm. CONCLUSIONS: Escherichia coli forms biofilms on static assays in a method-dependent fashion, depending on strain, and it is strongly modulated by culture conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: As verified in the studied E. coli strains, biofilm formation by any organism should be cautiously interpreted, considering all variables in the experimental settings.
AIMS: In this study, we have evaluated the impact of methodological approaches in the determination of biofilm formation by four clinical isolates of Escherichia coli in static assays. METHODS AND RESULTS: The assays were performed in microtitre plates with two minimal and two enriched broths, with one- or two-steps protocol, and using three different mathematical formulas to quantify adherent bacteria. Different biofilm formation patterns were found depending on the E. coli strain, culture medium and reading optical density on one- and two-steps protocol. Strong or moderate biofilm formation occurred mostly in minimal media. The mathematical formulas used to quantify biofilm formation also gave different results and bacterial growth rate should be taken into account to quantify biofilm. CONCLUSIONS:Escherichia coli forms biofilms on static assays in a method-dependent fashion, depending on strain, and it is strongly modulated by culture conditions. SIGNIFICANCE AND IMPACT OF THE STUDY: As verified in the studied E. coli strains, biofilm formation by any organism should be cautiously interpreted, considering all variables in the experimental settings.
Authors: Yien Shin Toh; Soo Ling Yeoh; Ivan Kok Seng Yap; Cindy Shuan Ju Teh; Thin Thin Win; Kwai Lin Thong; Chun Wie Chong Journal: Med Microbiol Immunol Date: 2019-07-01 Impact factor: 3.402
Authors: T Tapiainen; H Jauhiainen; L Jaakola; J Salo; J Sevander; I Ikäheimo; A M Pirttilä; A Hohtola; M Uhari Journal: Eur J Clin Microbiol Infect Dis Date: 2011-08-07 Impact factor: 3.267
Authors: Jiadong Sun; Jannie P J Marais; Christina Khoo; Kerry LaPlante; Rebecca M Vejborg; Michael Givskov; Tim Tolker-Nielsen; Navindra P Seeram; David C Rowley Journal: J Funct Foods Date: 2015-08 Impact factor: 4.451
Authors: Margarita Martinez-Medina; Plínio Naves; Jorge Blanco; Xavier Aldeguer; Jesus E Blanco; Miguel Blanco; Carmen Ponte; Francisco Soriano; Arlette Darfeuille-Michaud; L Jesus Garcia-Gil Journal: BMC Microbiol Date: 2009-09-21 Impact factor: 3.605