AIM: Vascular access is the lifeline of haemodialysis patients and radial-cephalic fistula is the preferred type of access. We investigated vascular calcification in uraemia radial arteries and compared it with clinical parameters. METHODS: Artery specimens from 30 end-stage renal disease patients were collected, examined calcification by von Kossa and Alizarin red staining. Expression of alpha-smooth muscle actin (alpha-SMA) and the main component of bone matrix, osteopontin (OP) were detected by immunohistochemistry. RESULTS: In uraemia vessels, calcification was mainly located in the medial layer. Nineteen (63.33%) patients had no evidence of calcification, six (20%) had mild/moderate calcification and five (16.66%) had severe calcification. Upregulation of OP and diminished expression of alpha-SMA occurred in the medial layer, especially in the area of severe calcification. The calcification score, decreased expression of alpha-SMA and upregulation of OP were positively correlated with older age, serum calcium, serum phosphorus and calcium x phosphorus product (P < 0.01). CONCLUSION: Vascular calcification in uraemia radial arteries is mainly located in the media layer. The risk factors appear to be older age, an elevated serum level of phosphorus, calcium and calcium x phosphorus product.
AIM: Vascular access is the lifeline of haemodialysis patients and radial-cephalic fistula is the preferred type of access. We investigated vascular calcification in uraemia radial arteries and compared it with clinical parameters. METHODS: Artery specimens from 30 end-stage renal diseasepatients were collected, examined calcification by von Kossa and Alizarin red staining. Expression of alpha-smooth muscle actin (alpha-SMA) and the main component of bone matrix, osteopontin (OP) were detected by immunohistochemistry. RESULTS: In uraemia vessels, calcification was mainly located in the medial layer. Nineteen (63.33%) patients had no evidence of calcification, six (20%) had mild/moderate calcification and five (16.66%) had severe calcification. Upregulation of OP and diminished expression of alpha-SMA occurred in the medial layer, especially in the area of severe calcification. The calcification score, decreased expression of alpha-SMA and upregulation of OP were positively correlated with older age, serum calcium, serum phosphorus and calcium x phosphorus product (P < 0.01). CONCLUSION:Vascular calcification in uraemia radial arteries is mainly located in the media layer. The risk factors appear to be older age, an elevated serum level of phosphorus, calcium and calcium x phosphorus product.
Authors: Anuja Shah; Clinton J Miller; Cynthia C Nast; Mark D Adams; Barbara Truitt; John A Tayek; Lili Tong; Parag Mehtani; Francisco Monteon; John R Sedor; Erica L Clinkenbeard; Kenneth White; Rajnish Mehrotra; Janine LaPage; Patricia Dickson; Sharon G Adler; Sudha K Iyengar Journal: Nephrol Dial Transplant Date: 2014-11-05 Impact factor: 5.992
Authors: Katarzyna Janda; Marcin Krzanowski; Mariusz Gajda; Paulina Dumnicka; Danuta Fedak; Grzegorz J Lis; Piotr Jaśkowski; Jan A Litwin; Władysław Sułowicz Journal: Int J Endocrinol Date: 2013-12-18 Impact factor: 3.257