| Literature DB >> 183628 |
H D Matheka, L Coggins, J N Shively, N L Norcross.
Abstract
EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5-30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20 degrees C) of 1.162 and a sedimentation constant of S20w=656. electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.Entities:
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Year: 1976 PMID: 183628 DOI: 10.1007/bf01317839
Source DB: PubMed Journal: Arch Virol ISSN: 0304-8608 Impact factor: 2.574