Literature DB >> 18360812

Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma.

Federica Alciato1, Pier Paolo Sainaghi, Luigi Castello, Luca Bergamasco, Stefania Carnieletto, Gian Carlo Avanzi.   

Abstract

Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was prepared to optimize the assay. The ELISA method showed inter- and intra-assay coefficients of variation lower than 15%. Recoveries all fell within 15% of expected values. Plasma Gas6 concentration in 61 healthy donors was 20.3+/-3.8 ng/mL. Our assay meets FDA requirements for precision and accuracy for the validation of bioanalytical methods and it is suitable for research or diagnostic purposes.

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Year:  2008        PMID： 18360812     DOI： 10.1080/15321810801888480

Source DB:  PubMed          Journal:  J Immunoassay Immunochem        ISSN： 1532-1819


  14 in total

1.  Elevated plasma gas6 levels are associated with venous thromboembolic disease.

Authors:  Mark D Blostein; Isabelle Rajotte; Deepa P Rao; Christina A Holcroft; Susan R Kahn
Journal:  J Thromb Thrombolysis       Date:  2011-10       Impact factor: 2.300

2.  Elevated serum level of growth arrest-specific protein 6 (Gas6) in systemic lupus erythematosus patients is associated with nephritis and cutaneous vasculitis.

Authors:  Chien-Sheng Wu; Chung-Yi Hu; Hwei-Fang Tsai; I-Tsu Chyuan; Cheng-Ju Chan; Sheng-Kai Chang; Ping-Ning Hsu
Journal:  Rheumatol Int       Date:  2013-11-01       Impact factor: 2.631

3.  TAM receptor ligands in lupus: protein S but not Gas6 levels reflect disease activity in systemic lupus erythematosus.

Authors:  Chang-Hee Suh; Brendan Hilliard; Sophia Li; Joan T Merrill; Philip L Cohen
Journal:  Arthritis Res Ther       Date:  2010-07-16       Impact factor: 5.156

4.  Plasma protein growth arrest-specific 6 levels are associated with altered glucose tolerance, inflammation, and endothelial dysfunction.

Authors:  Yi-Jen Hung; Chien-Hsing Lee; Nain-Feng Chu; Yi-Shing Shieh
Journal:  Diabetes Care       Date:  2010-05-26       Impact factor: 19.112

5.  Novel Axl-driven signaling pathway and molecular signature characterize high-grade ovarian cancer patients with poor clinical outcome.

Authors:  Katia Rea; Patrizia Pinciroli; Marialuisa Sensi; Federica Alciato; Brigitte Bisaro; Ludmila Lozneanu; Francesco Raspagliesi; Floriana Centritto; Sara Cabodi; Paola Defilippi; Gian Carlo Avanzi; Silvana Canevari; Antonella Tomassetti
Journal:  Oncotarget       Date:  2015-10-13

Review 6.  The Gas6/TAM System and Multiple Sclerosis.

Authors:  Mattia Bellan; Mario Pirisi; Pier Paolo Sainaghi
Journal:  Int J Mol Sci       Date:  2016-10-28       Impact factor: 5.923

7.  Growth arrest specific gene 6 protein concentration in cerebrospinal fluid correlates with relapse severity in multiple sclerosis.

Authors:  P P Sainaghi; L Collimedaglia; F Alciato; R Molinari; D Sola; E Ranza; P Naldi; F Monaco; M Leone; M Pirisi; G C Avanzi
Journal:  Mediators Inflamm       Date:  2013-05-27       Impact factor: 4.711

8.  Vitamin K-dependent proteins GAS6 and Protein S and TAM receptors in patients of systemic lupus erythematosus: correlation with common genetic variants and disease activity.

Authors:  Pedro Recarte-Pelz; Dolors Tàssies; Gerard Espinosa; Begoña Hurtado; Núria Sala; Ricard Cervera; Joan Carles Reverter; Pablo García de Frutos
Journal:  Arthritis Res Ther       Date:  2013-03-12       Impact factor: 5.156

9.  Growth Arrest-Specific 6 Protein in Patients with Sjögren Syndrome: Determination of the Plasma Level and Expression in the Labial Salivary Gland.

Authors:  Chen-Hung Chen; Hsiang-Cheng Chen; Chi-Ching Chang; Yi-Jen Peng; Chien-Hsing Lee; Yi-Shing Shieh; Yi-Jen Hung; Yuh-Feng Lin
Journal:  PLoS One       Date:  2015-10-07       Impact factor: 3.240

10.  Effect of GAS6 and AXL Gene Polymorphisms on Adiposity, Systemic Inflammation, and Insulin Resistance in Adolescents.

Authors:  Fone-Ching Hsiao; Yuh-Feng Lin; Po-Shiuan Hsieh; Nain-Feng Chu; Yii-Der Ida Chen; Yi-Shing Shieh; Chang-Hsun Hsieh; Chien-Hsing Lee; Ting-I Lee; Yi-Jen Hung
Journal:  Int J Endocrinol       Date:  2014-02-18       Impact factor: 3.257
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