AIM: To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion. METHODS: The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber. RESULTS: p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a human leukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing human P-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner. CONCLUSIONS: p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.
AIM: To investigate the anti-inflammatory mechanism of the polysaccharides of Ginkgo biloba leaves (PGBL) by inhibiting leucocyte adhesion. METHODS: The rough PGBL were isolated and purified. The anti-inflammatory effects of purified PGBL (p-PGBL) were assayed by ear edema induced by xylol and the acute peritonitis model in mice. The effect of p-PGBL on inhibiting the interaction between P-selectin and its ligands was investigated by flow cytometry and flow chamber. RESULTS:p-PGBL could effectively inhibit the acute inflammation in mice and interfere with the adhesion of HL-60 cells, a humanleukaemia cell line, or neutrophils to P-selectin in static conditions, as well as the adhesion of neutrophils to Chinese hamster ovary cells expressing humanP-selectin and human umbilical vein endothelial cells in flow conditions in a dose-dependant manner. CONCLUSIONS:p-PGBL can inhibit the inflammatory process through interfering with the interaction between P-selectin and its ligands.
Authors: Viera Jančinová; Tomáš Perečko; Rado Nosáľ; Juraj Harmatha; Jan Smidrkal; Katarína Drábiková Journal: Acta Pharmacol Sin Date: 2012-07-30 Impact factor: 6.150