AIMS: We determined if the intra-specific genetic diversity of Microcystis aeruginosa correlates with phenotypic characteristics. METHODS AND RESULTS: Microcystis aeruginosa isolates from various Japanese waters were characterized using genetic analyses based on the 16S-23S rDNA internal transcribed spacer (ITS) region and DNA-independent RNA polymerase (rpoC1) gene sequences. In addition, morphological and biochemical properties, and the toxicity of M. aeruginosa strains were determined. We found a correlation in phylogenetic clusters of the ITS region and rpoC1 gene sequences. Using a polyphasic approach, genotypic and phenotypic variations in M. aeruginosa showed that the three genetic lineage groups are comprised of a particular phenotype or subgroup of closely related phenotypes. However, some strains had high phenotypic and genotypic diversity compared to the three lineage groups and did not show distinct lineages; therefore, these strains were designated as the 'complex group'. CONCLUSIONS: The 'complex group' consisted of genetically and phenotypically incoherent and high diverse populations in M. aeruginosa, although some genotypes or lineages displayed consistent phenotypes. SIGNIFICANCE AND IMPACT OF THE STUDY: The polyphasic approach combining phenotypic and genetic characterization was effective for comprehending distinct lineages and discriminating the potential complexity of M. aeruginosa populations at the intra-species level.
AIMS: We determined if the intra-specific genetic diversity of Microcystis aeruginosa correlates with phenotypic characteristics. METHODS AND RESULTS:Microcystis aeruginosa isolates from various Japanese waters were characterized using genetic analyses based on the 16S-23S rDNA internal transcribed spacer (ITS) region and DNA-independent RNA polymerase (rpoC1) gene sequences. In addition, morphological and biochemical properties, and the toxicity of M. aeruginosa strains were determined. We found a correlation in phylogenetic clusters of the ITS region and rpoC1 gene sequences. Using a polyphasic approach, genotypic and phenotypic variations in M. aeruginosa showed that the three genetic lineage groups are comprised of a particular phenotype or subgroup of closely related phenotypes. However, some strains had high phenotypic and genotypic diversity compared to the three lineage groups and did not show distinct lineages; therefore, these strains were designated as the 'complex group'. CONCLUSIONS: The 'complex group' consisted of genetically and phenotypically incoherent and high diverse populations in M. aeruginosa, although some genotypes or lineages displayed consistent phenotypes. SIGNIFICANCE AND IMPACT OF THE STUDY: The polyphasic approach combining phenotypic and genetic characterization was effective for comprehending distinct lineages and discriminating the potential complexity of M. aeruginosa populations at the intra-species level.
Authors: Olga M Pérez-Carrascal; Yves Terrat; Alessandra Giani; Nathalie Fortin; Charles W Greer; Nicolas Tromas; B Jesse Shapiro Journal: ISME J Date: 2019-07-30 Impact factor: 10.302
Authors: Ineke van Gremberghe; Frederik Leliaert; Joachim Mergeay; Pieter Vanormelingen; Katleen Van der Gucht; Ann-Eline Debeer; Gissell Lacerot; Luc De Meester; Wim Vyverman Journal: PLoS One Date: 2011-05-05 Impact factor: 3.240