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Literature DB >> 18353106

Expression of a mutated phospholipase A2 in transgenic Aedes fluviatilis mosquitoes impacts Plasmodium gallinaceum development.

F G Rodrigues¹, M N Santos, T X T de Carvalho, B C Rocha, M A Riehle, P F P Pimenta, E G Abraham, M Jacobs-Lorena, C F Alves de Brito, L A Moreira.

Abstract

The genetic manipulation of mosquito vectors is an alternative strategy in the fight against malaria. It was previously shown that bee venom phospholipase A2 (PLA2) inhibits ookinete invasion of the mosquito midgut although mosquito fitness was reduced. To maintain the PLA2 blocking ability without compromising mosquito biology, we mutated the protein-coding sequence to inactivate the enzyme while maintaining the protein's structure. DNA encoding the mutated PLA2 (mPLA2) was placed downstream of a mosquito midgut-specific promoter (Anopheles gambiae peritrophin protein 1 promoter, AgPer1) and this construct used to transform Aedes fluviatilis mosquitoes. Four different transgenic lines were obtained and characterized and all lines significantly inhibited Plasmodium gallinaceum oocyst development (up to 68% fewer oocysts). No fitness cost was observed when this mosquito species expressed the mPLA2.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 2008 PMID： 18353106 PMCID： PMC4137777 DOI： 10.1111/j.1365-2583.2008.00791.x

Source DB: PubMed Journal: Insect Mol Biol ISSN： 0962-1075 Impact factor: 3.585

41 in total

1. Targeting Plasmodium ligands on mosquito salivary glands and midgut with a phage display peptide library.

Authors: A K Ghosh; P E Ribolla; M Jacobs-Lorena
Journal: Proc Natl Acad Sci U S A Date: 2001-10-30 Impact factor: 11.205

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Journal: J Biol Chem Date: 2001-11-02 Impact factor: 5.157

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Journal: Am J Trop Med Hyg Date: 2000-04 Impact factor: 2.345

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Authors: C Deregnaucourt; J Schrével
Journal: J Biol Chem Date: 2000-12-22 Impact factor: 5.157

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Authors: A G Buckland; D C Wilton
Journal: Biochim Biophys Acta Date: 2000-10-31

8. Efficient transformation of the yellow fever mosquito Aedes aegypti using the piggyBac transposable element vector pBac[3xP3-EGFP afm].

Authors: V Kokoza; A Ahmed; E A Wimmer; A S Raikhel
Journal: Insect Biochem Mol Biol Date: 2001-11-01 Impact factor: 4.714

9. Transgenic anopheline mosquitoes impaired in transmission of a malaria parasite.

Authors: Junitsu Ito; Anil Ghosh; Luciano A Moreira; Ernst A Wimmer; Marcelo Jacobs-Lorena
Journal: Nature Date: 2002-05-23 Impact factor: 49.962

10. A snake venom phospholipase A(2) blocks malaria parasite development in the mosquito midgut by inhibiting ookinete association with the midgut surface.

Authors: H Zieler; D B Keister; J A Dvorak; J M Ribeiro
Journal: J Exp Biol Date: 2001-12 Impact factor: 3.312

13 in total

Review 1. Safe and fit genetically modified insects for pest control: from lab to field applications.

Authors: F Scolari; P Siciliano; P Gabrieli; L M Gomulski; A Bonomi; G Gasperi; A R Malacrida
Journal: Genetica Date: 2010-08-20 Impact factor: 1.082

Review 2. Gene expression studies in mosquitoes.

Authors: Xiao-Guang Chen; Geetika Mathur; Anthony A James
Journal: Adv Genet Date: 2008 Impact factor: 1.944

3. Fitness aspects of transgenic Aedes fluviatilis mosquitoes expressing a Plasmodium-blocking molecule.

Authors: Maíra N Santos; Paula M Nogueira; Fernando B S Dias; Denise Valle; Luciano A Moreira
Journal: Transgenic Res Date: 2010-02-13 Impact factor: 2.788

Review 4. Patatin-like phospholipases in microbial infections with emerging roles in fatty acid metabolism and immune regulation by Apicomplexa.

Authors: Sarah K Wilson; Laura J Knoll
Journal: Mol Microbiol Date: 2017-11-23 Impact factor: 3.501

10. Contrasted Fitness Costs of Docking and Antibacterial Constructs in the EE and EVida3 Strains Validates Two-Phase Anopheles gambiae Genetic Transformation System.

Authors: Doug Paton; Anne Underhill; Janet Meredith; Paul Eggleston; Frederic Tripet
Journal: PLoS One Date: 2013-06-26 Impact factor: 3.240