BACKGROUND: Hypothermic perfusion (HP) of the liver is applied during total vascular exclusion (TVE) to reduce ischemic injury during liver resection. No studies have been performed comparing different perfusion solutions for HP. The aim of this experimental study was to compare Ringer-lactate solution (RL) with Celsior solution (Cs) for HP in a pig model of 60-min TVE. METHOD: Twenty pigs underwent 60-min TVE of the liver. Groups were TVE without HP (no-HP, n = 9), TVE with HP using RL (n = 6), and TVE with HP using Cs (n = 5). Blood and liver tissue samples were taken before TVE and during 24-h reperfusion. RESULTS: In the no-HP group, plasma aspartate aminotransferase values were significantly increased during reperfusion (p < 0.05), while liver tissue pO(2) levels (p < 0.01) were decreased when compared to the HP groups. After 24-h reperfusion, bile production and liver tissue glutathione content were significantly higher (p < 0.05) in the Cs group (42.0 +/- 1.7 mL/h and 44.9 +/- 2.2 nmol/mg, respectively) as compared to the RL group (31.5 +/- 3.5 mL/h and 19.6 +/- 1.8 nmol/mg, respectively). CONCLUSION: The protective effect of HP during TVE was confirmed in this study. HP with Cs was more effective in reducing ischemic injury as compared to HP with RL.
BACKGROUND: Hypothermic perfusion (HP) of the liver is applied during total vascular exclusion (TVE) to reduce ischemic injury during liver resection. No studies have been performed comparing different perfusion solutions for HP. The aim of this experimental study was to compare Ringer-lactate solution (RL) with Celsior solution (Cs) for HP in a pig model of 60-min TVE. METHOD: Twenty pigs underwent 60-min TVE of the liver. Groups were TVE without HP (no-HP, n = 9), TVE with HP using RL (n = 6), and TVE with HP using Cs (n = 5). Blood and liver tissue samples were taken before TVE and during 24-h reperfusion. RESULTS: In the no-HP group, plasma aspartate aminotransferase values were significantly increased during reperfusion (p < 0.05), while liver tissue pO(2) levels (p < 0.01) were decreased when compared to the HP groups. After 24-h reperfusion, bile production and liver tissue glutathione content were significantly higher (p < 0.05) in the Cs group (42.0 +/- 1.7 mL/h and 44.9 +/- 2.2 nmol/mg, respectively) as compared to the RL group (31.5 +/- 3.5 mL/h and 19.6 +/- 1.8 nmol/mg, respectively). CONCLUSION: The protective effect of HP during TVE was confirmed in this study. HP with Cs was more effective in reducing ischemic injury as compared to HP with RL.
Authors: Bob H M Heijnen; Irene H Straatsburg; Liesbeth M Kager; Ad J van der Kleij; Dirk J Gouma; Thomas M van Gulik Journal: Liver Int Date: 2003-02 Impact factor: 5.828
Authors: Paola Pedotti; Massimo Cardillo; Paolo Rigotti; Giorgio Gerunda; Roberto Merenda; Umberto Cillo; Giacomo Zanus; Umberto Baccarani; Maria Luisa Berardinelli; Luigi Boschiero; Lucio Caccamo; Gilberto Calconi; Stefano Chiaramonte; Antonio Dal Canton; Luciano De Carlis; Valerio Di Carlo; Donato Donati; Domenico Montanaro; Andrea Pulvirenti; Giuseppe Remuzzi; Silvio Sandrini; Umberto Valente; Mario Scalamogna Journal: Transplantation Date: 2004-05-27 Impact factor: 4.939
Authors: Rolf J Schauer; Alexander L Gerbes; Daniel Vonier; Herbert Meissner; Patrick Michl; Rosemarie Leiderer; Friedrich W Schildberg; Konrad Messmer; Manfred Bilzer Journal: Ann Surg Date: 2004-02 Impact factor: 12.969