Literature DB >> 18349361

PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences.

Hsiao-Jan Chen1, Jui-Chang Tsai2,3, Tsung-Chain Chang4, Wei-Chun Hung1, Sung-Pin Tseng1, Po-Ren Hsueh5, Lee-Jene Teng5,1.   

Abstract

The sequence diversity of groESL genes among Streptococcus bovis group isolates was analysed, including five reference strains and 36 clinical isolates. Phylogenetic analysis of the groES and groEL sequences showed that the isolates that belonged to the same species or subspecies usually clustered together. The intergenic spacer region between groES and groEL was variable in size (67-342 bp) and sequence and appeared to be a unique marker for species or subspecies determination. Sequence similarities of the groESL genes among species and subspecies ranged from 84.2 to 99.0 % in groES, and from 88.0 to 99.0 % in groEL. Based on the sequences determined, a Streptococcus bovis group-specific PCR assay was developed, which may provide an alternative means of distinguishing the bovis group from other viridans streptococci. Restriction digestion of the amplicon with AclI further differentiated the species and subspecies.

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Year:  2008        PMID： 18349361     DOI： 10.1099/jmm.0.47628-0

Source DB:  PubMed          Journal:  J Med Microbiol        ISSN： 0022-2615            Impact factor:   2.472


  7 in total

1.  Use of groESL as a target for identification of Abiotrophia, Granulicatella, and Gemella species.

Authors:  Wei-Chun Hung; Sung-Pin Tseng; Hsiao-Jan Chen; Jui-Chang Tsai; Chih-Hsin Chang; Tai-Fen Lee; Po-Ren Hsueh; Lee-Jene Teng
Journal:  J Clin Microbiol       Date:  2010-08-04       Impact factor: 5.948

2.  Novel real-time PCR assays using TaqMan minor groove binder probes for identification of fecal carriage of Streptococcus bovis/Streptococcus equinus complex from rectal swab specimens.

Authors:  Paulo Guilherme Markus Lopes; Vlademir Vicente Cantarelli; Grasiela Agnes; Ane Micheli Costabeber; Pedro Alves d'Azevedo
Journal:  J Clin Microbiol       Date:  2014-01-03       Impact factor: 5.948

3.  Development of real-time PCR assays for detection of the Streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16S rRNA genes.

Authors:  A B Olson; C D Sibley; L Schmidt; M A Wilcox; M G Surette; C R Corbett
Journal:  J Clin Microbiol       Date:  2010-02-17       Impact factor: 5.948

4.  The relationship between the new taxonomy of Streptococcus bovis and its clonality to colon cancer, endocarditis, and biliary disease.

Authors:  T Lazarovitch; M Shango; M Levine; R Brusovansky; R Akins; K Hayakawa; P R Lephart; J D Sobel; K S Kaye; D Marchaim
Journal:  Infection       Date:  2012-08-11       Impact factor: 3.553

5.  Prevalence and comparison of Streptococcus infantarius subsp. infantarius and Streptococcus gallolyticus subsp. macedonicus in raw and fermented dairy products from East and West Africa.

Authors:  Christoph Jans; Dasel Wambua Mulwa Kaindi; Désirée Böck; Patrick Murigu Kamau Njage; Sylvie Mireille Kouamé-Sina; Bassirou Bonfoh; Christophe Lacroix; Leo Meile
Journal:  Int J Food Microbiol       Date:  2013-09-21       Impact factor: 5.277

6.  Phylogenetic, epidemiological and functional analyses of the Streptococcus bovis/Streptococcus equinus complex through an overarching MLST scheme.

Authors:  Christoph Jans; Tomas de Wouters; Bassirou Bonfoh; Christophe Lacroix; Dasel Wambua Mulwa Kaindi; Janine Anderegg; Désirée Böck; Sabrina Vitali; Thomas Schmid; Julia Isenring; Fabienne Kurt; Wambui Kogi-Makau; Leo Meile
Journal:  BMC Microbiol       Date:  2016-06-21       Impact factor: 3.605

Review 7.  The Road to Infection: Host-Microbe Interactions Defining the Pathogenicity of Streptococcus bovis/Streptococcus equinus Complex Members.

Authors:  Christoph Jans; Annemarie Boleij
Journal:  Front Microbiol       Date:  2018-04-10       Impact factor: 5.640
  7 in total

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