Jun Wang1, Xiu-Mei Liu. 1. Institute of Nutrition and Food Safety, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Abstract
OBJECTIVE: To study the contamination of total aflatoxins (AFs) in different kinds of foods including corn, peanut, rice, walnut, and pine nut in six provinces and two municipalities in China. METHODS: A total of 283 samples of corn, peanut, rice, walnut and pine nut were randomly collected from local markets in Fujian, Guangdong, Guangxi, Hubei, Jiangsu, and Zhejiang provinces, as well as in Shanghai and Chongqing municipalities. The samples were ground to which acetonitrile/water solution was added. After filtering, the extract was transferred into a MycoSep purifying column and was pressed slowly. Then the purified liquid was derivatized with trifluoroacetic acid (TFA) and assayed using high performance liquid chromatography (HPLC). RESULTS: AFs were detected in 70.27% of corn samples, with a mean level of 27.44 microg/kg and the highest level of 1098.36 microg/kg. In peanut, the AFs detection rate was 23.08%, with a mean level of 0.82 microg/kg and the highest level of 28.39 microg/kg. Very few rice samples with AFs were detected. The AFs levels were very low in walnut and pine nut. CONCLUSION: Corn is the food most seriously contaminated with AFs in China. AFB1 is the main aflatoxin which is found as a contaminant in foods.
OBJECTIVE: To study the contamination of total aflatoxins (AFs) in different kinds of foods including corn, peanut, rice, walnut, and pine nut in six provinces and two municipalities in China. METHODS: A total of 283 samples of corn, peanut, rice, walnut and pine nut were randomly collected from local markets in Fujian, Guangdong, Guangxi, Hubei, Jiangsu, and Zhejiang provinces, as well as in Shanghai and Chongqing municipalities. The samples were ground to which acetonitrile/water solution was added. After filtering, the extract was transferred into a MycoSep purifying column and was pressed slowly. Then the purified liquid was derivatized with trifluoroacetic acid (TFA) and assayed using high performance liquid chromatography (HPLC). RESULTS: AFs were detected in 70.27% of corn samples, with a mean level of 27.44 microg/kg and the highest level of 1098.36 microg/kg. In peanut, the AFs detection rate was 23.08%, with a mean level of 0.82 microg/kg and the highest level of 28.39 microg/kg. Very few rice samples with AFs were detected. The AFs levels were very low in walnut and pine nut. CONCLUSION:Corn is the food most seriously contaminated with AFs in China. AFB1 is the main aflatoxin which is found as a contaminant in foods.
Authors: J Bansal; P Pantazopoulos; J Tam; P Cavlovic; K Kwong; A-M Turcotte; B P-Y Lau; P M Scott Journal: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Date: 2011-06