A Sepe1, P Barbieri, R Peduzzi, A Demarta. 1. Laboratory of Environmental Microbiology, Istituto Cantonale di Microbiologia, Bellinzona, Switzerland, and Università degli Studi dell'Insubria, Varese, Italy.
Abstract
AIMS: To evaluate the usefulness of partial recA sequences for the identification of Aeromonas strains at the genotype level. METHODS AND RESULTS: A partial recA sequence was obtained from 21 type or reference strains and 33 Aeromonas isolates, collected in the South of Switzerland from human, animal and aquatic environments. The 272 bp long recA fragments showed a mean interspecies divergence of 7.8% and allowed the classification of strains at genotype level. However, some discrepancies could be observed with other gene sequence based analyses in the classification of some strains. CONCLUSIONS: The 272 bp long recA fragment is a good molecular marker to infer taxonomy of members of the genus Aeromonas, even if the primers we chose for the amplification did not allow its direct sequencing. SIGNIFICANCE AND IMPACT OF THE STUDY: In the genus Aeromonas, nucleotide sequences of some protein-encoding genes have already been evaluated as molecular markers to be used in taxonomical and epidemiological researches. This study suggests the usefulness of a recA fragment as a further sequence to investigate for these purposes.
AIMS: To evaluate the usefulness of partial recA sequences for the identification of Aeromonas strains at the genotype level. METHODS AND RESULTS: A partial recA sequence was obtained from 21 type or reference strains and 33 Aeromonas isolates, collected in the South of Switzerland from human, animal and aquatic environments. The 272 bp long recA fragments showed a mean interspecies divergence of 7.8% and allowed the classification of strains at genotype level. However, some discrepancies could be observed with other gene sequence based analyses in the classification of some strains. CONCLUSIONS: The 272 bp long recA fragment is a good molecular marker to infer taxonomy of members of the genus Aeromonas, even if the primers we chose for the amplification did not allow its direct sequencing. SIGNIFICANCE AND IMPACT OF THE STUDY: In the genus Aeromonas, nucleotide sequences of some protein-encoding genes have already been evaluated as molecular markers to be used in taxonomical and epidemiological researches. This study suggests the usefulness of a recA fragment as a further sequence to investigate for these purposes.
Authors: Tien Tien Vicky Lau; Jin-Ai Mary Anne Tan; S D Puthucheary; Suat-Moi Puah; Kek-Heng Chua Journal: Braz J Microbiol Date: 2020-08-07 Impact factor: 2.476
Authors: Adam C Silver; David Williams; Joshua Faucher; Amy J Horneman; J Peter Gogarten; Joerg Graf Journal: PLoS One Date: 2011-02-16 Impact factor: 3.240