Literature DB >> 1834524

A pgk::hprt fusion as a selectable marker for targeting of genes in mouse embryonic stem cells: disruption of the T-cell receptor delta-chain-encoding gene.

N van der Lugt1, E R Maandag, H te Riele, P W Laird, A Berns.   

Abstract

We have constructed a hypoxanthine phosphoribosyl transferase-selectable marker (hprt) under the control of the phosphoglycerate kinase (pgk) promoter. This construct permits cell growth in hypoxanthine/aminopterin/thymidine media and confers 6-thioguanine sensitivity upon mouse Hprt- embryonic stem cells, allowing either positive or negative selection in gene-targeting experiments. We have successfully targeted the gene encoding the T-cell receptor delta-chain using the pgk::hprt fusion for counterselection.

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Year:  1991        PMID： 1834524     DOI： 10.1016/0378-1119(91)90161-4

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  8 in total

1.  Fatal embryonic bleeding events in mice lacking tissue factor, the cell-associated initiator of blood coagulation.

Authors:  T H Bugge; Q Xiao; K W Kombrinck; M J Flick; K Holmbäck; M J Danton; M C Colbert; D P Witte; K Fujikawa; E W Davie; J L Degen
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-25       Impact factor: 11.205

2.  Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs.

Authors:  H te Riele; E R Maandag; A Berns
Journal:  Proc Natl Acad Sci U S A       Date:  1992-06-01       Impact factor: 11.205

3.  Complementation of methylation deficiency in embryonic stem cells by a DNA methyltransferase minigene.

Authors:  K L Tucker; D Talbot; M A Lee; H Leonhardt; R Jaenisch
Journal:  Proc Natl Acad Sci U S A       Date:  1996-11-12       Impact factor: 11.205

4.  Biological effects of targeted inactivation of hepatocyte growth factor-like protein in mice.

Authors:  J A Bezerra; T L Carrick; J L Degen; D Witte; S J Degen
Journal:  J Clin Invest       Date:  1998-03-01       Impact factor: 14.808

5.  Transgenic mice for the preparation of hygromycin-resistant primary embryonic fibroblast feeder layers for embryonic stem cell selections.

Authors:  K A Johnson; C P Lerner; L C Di Lacio; P W Laird; A H Sharpe; E M Simpson
Journal:  Nucleic Acids Res       Date:  1995-04-11       Impact factor: 16.971

6.  Impaired platelet aggregation and sustained bleeding in mice lacking the fibrinogen motif bound by integrin alpha IIb beta 3.

Authors:  K Holmbäck; M J Danton; T T Suh; C C Daugherty; J L Degen
Journal:  EMBO J       Date:  1996-11-01       Impact factor: 11.598

Review 7.  Mouse genetics in the 21st century: using gene targeting to create a cornucopia of mouse mutants possessing precise genetic modifications.

Authors:  P J Wilder; A Rizzino
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

8.  Gsh-1, an orphan Hox gene, is required for normal pituitary development.

Authors:  H Li; P S Zeitler; M T Valerius; K Small; S S Potter
Journal:  EMBO J       Date:  1996-02-15       Impact factor: 11.598
  8 in total

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