PURPOSE: To investigate the kinetics and the determinants of the changes of the Clara cell 16 kDa protein (CC16) as a marker of lung injury following exposure to different toxicants. METHODS: Rats were treated with 4-Ipomeanol (IPO), alpha-naphtylthiourea (ANTU), sodium chromate (Na(2)CrO(4)) or paraquat-(PQ). The changes of CC16, total protein, albumin, and cystatin-C were determined on BALF supernatan, and CC16, cystatin-C and creatinine levels were also determined in serum. RESULTS: Bronchiolar insult due to IPO or ANTU resulted in an initial transient increase of serum CC16, parallel to that of albumin in BALF, and a subsequent reduction in both BALF and serum. A slight reduction of CC16 in BALF was already apparent one hour following treatment with PQ. In the serum, CC16 increased to 400% of basal value. With PQ and Na(2)CrO(4), the elevation of CC16 was mainly determined by the degree of renal impairment.
PURPOSE: To investigate the kinetics and the determinants of the changes of the Clara cell 16 kDa protein (CC16) as a marker of lung injury following exposure to different toxicants. METHODS:Rats were treated with 4-Ipomeanol (IPO), alpha-naphtylthiourea (ANTU), sodium chromate (Na(2)CrO(4)) or paraquat-(PQ). The changes of CC16, total protein, albumin, and cystatin-C were determined on BALF supernatan, and CC16, cystatin-C and creatinine levels were also determined in serum. RESULTS: Bronchiolar insult due to IPO or ANTU resulted in an initial transient increase of serum CC16, parallel to that of albumin in BALF, and a subsequent reduction in both BALF and serum. A slight reduction of CC16 in BALF was already apparent one hour following treatment with PQ. In the serum, CC16 increased to 400% of basal value. With PQ and Na(2)CrO(4), the elevation of CC16 was mainly determined by the degree of renal impairment.
Authors: Gideon St Helen; Nina T Holland; John R Balmes; Daniel B Hall; J Thomas Bernert; John E Vena; Jia-Sheng Wang; Luke P Naeher Journal: J Expo Sci Environ Epidemiol Date: 2012-07-18 Impact factor: 5.563
Authors: Walter C Prozialeck; Joshua R Edwards; Vishal S Vaidya; Joseph V Bonventre Journal: Toxicol Appl Pharmacol Date: 2009-02-06 Impact factor: 4.219
Authors: Darren M Roberts; Martin F Wilks; Michael S Roberts; Ramasamyiyer Swaminathan; Fahim Mohamed; Andrew H Dawson; Nick A Buckley Journal: Toxicol Lett Date: 2011-02-01 Impact factor: 4.372