Literature DB >> 18343402

Detection and quantitation of Solenopsis invicta virus-2 genomic and intermediary replicating viral RNA in fire ant workers and larvae.

Yoshifumi Hashimoto1, Steven M Valles.   

Abstract

Quantitative real-time PCR (QPCR) was used to quantify the genome of Solenopsis invicta virus-2 (SINV-2) from infected individual ants of S. invicta. Strand-specific cDNA synthesis oligonucleotide primers and RNase digestion after cDNA synthesis allowed quantification of plus (genomic) and minus (replicative) strands of the SINV-2 genome. Both strands were detected in adult workers and larval fire ants indicating that the virus was replicating within the ant. The differences between the genomic to replicative strand ranged from 199-fold in larvae to 479-fold in workers with an average ratio of 339:1.

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Year:  2008        PMID: 18343402     DOI: 10.1016/j.jip.2008.02.002

Source DB:  PubMed          Journal:  J Invertebr Pathol        ISSN: 0022-2011            Impact factor:   2.841


  2 in total

1.  Metatranscriptomics and pyrosequencing facilitate discovery of potential viral natural enemies of the invasive Caribbean crazy ant, Nylanderia pubens.

Authors:  Steven M Valles; David H Oi; Fahong Yu; Xin-Xing Tan; Eileen A Buss
Journal:  PLoS One       Date:  2012-02-27       Impact factor: 3.240

2.  Accurate strand-specific quantification of viral RNA.

Authors:  Nicole E Plaskon; Zach N Adelman; Kevin M Myles
Journal:  PLoS One       Date:  2009-10-22       Impact factor: 3.240

  2 in total

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