| Literature DB >> 18343231 |
D Monchaud1, C Allain, H Bertrand, N Smargiasso, F Rosu, V Gabelica, A De Cian, J-L Mergny, M-P Teulade-Fichou.
Abstract
We report here the details of G4-FID (G-quadruplex fluorescent intercalator displacement), a simple method aiming at evaluating quadruplex-DNA binding affinity and quadruplex- over duplex-DNA selectivity of putative ligands. This assay is based on the loss of fluorescence upon displacement of thiazole orange from quadruplex- and duplex-DNA matrices. The original protocol was tested using various quadruplex- and duplex-DNA targets, and with a wide panel of G-quadruplex ligands belonging to different families (i.e. from quinacridines to metallo-organic ligands) likely to display various binding modes. The reliability of the assay is further supported by comparisons with FRET-melting and ESI-MS assays.Entities:
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Year: 2008 PMID： 18343231 DOI： 10.1016/j.biochi.2008.02.019
Source DB: PubMed Journal: Biochimie ISSN： 0300-9084 Impact factor: 4.079