OBJECTIVES: We examined the expression of Rho-kinase (ROK) isoforms in young and old rabbits' detrusor smooth muscles (SM) during the progression of short-term partial bladder outlet obstruction and correlated them with the time course of obstruction. METHODS: We obtained detrusor samples from bladders after 1, 3, 7, and 14 days of obstruction and also sham-operated control rabbits. We used reverse transcriptase-polymerase chain reaction, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blotting to determine the relative levels of ROK isoform expression at the mRNA and protein levels. RESULTS: Bladder weight for young rabbits increased between 1 and 7 days' obstruction and came back toward control levels at 14 days' obstruction. In old rabbits, bladder weight increased after obstruction, reaching a maximum at 3 days and remaining at this level throughout the 14 days. In young rabbits, the expression of ROKalpha increased in 1- to 7-day obstructed groups and decreased in the 14-day group, whereas it increased progressively in the old rabbits at both the mRNA and protein levels. There was a significant decrease in the expression of ROKbeta in young obstructed rabbits, which gradually decreased during the course of 1- to 7-day obstruction period and increased after 14 days of obstruction. In old groups, there was a decrease in expression after 1 day of obstruction and values remained at a decreased level throughout the course of the study. CONCLUSIONS: Young rabbit bladders are better able to adapt to bladder outlet obstruction and ROK isoforms respond in a similar way.
OBJECTIVES: We examined the expression of Rho-kinase (ROK) isoforms in young and old rabbits' detrusor smooth muscles (SM) during the progression of short-term partial bladder outlet obstruction and correlated them with the time course of obstruction. METHODS: We obtained detrusor samples from bladders after 1, 3, 7, and 14 days of obstruction and also sham-operated control rabbits. We used reverse transcriptase-polymerase chain reaction, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and Western blotting to determine the relative levels of ROK isoform expression at the mRNA and protein levels. RESULTS: Bladder weight for young rabbits increased between 1 and 7 days' obstruction and came back toward control levels at 14 days' obstruction. In old rabbits, bladder weight increased after obstruction, reaching a maximum at 3 days and remaining at this level throughout the 14 days. In young rabbits, the expression of ROKalpha increased in 1- to 7-day obstructed groups and decreased in the 14-day group, whereas it increased progressively in the old rabbits at both the mRNA and protein levels. There was a significant decrease in the expression of ROKbeta in young obstructed rabbits, which gradually decreased during the course of 1- to 7-day obstruction period and increased after 14 days of obstruction. In old groups, there was a decrease in expression after 1 day of obstruction and values remained at a decreased level throughout the course of the study. CONCLUSIONS: Young rabbit bladders are better able to adapt to bladder outlet obstruction and ROK isoforms respond in a similar way.
Authors: Ahmet Guven; Carmin Kalorin; Bulent Onal; Catherine Whitbeck; Paul Chichester; Barry A Kogan; Robert M Levin; Anita Mannikarottu Journal: Neurourol Urodyn Date: 2007 Impact factor: 2.696
Authors: Wu Bing; Shaohua Chang; Joseph A Hypolite; Michael E DiSanto; Stephen A Zderic; Lester Rolf; Alan J Wein; Samuel Chacko Journal: Am J Physiol Renal Physiol Date: 2003-07-08
Authors: Ettickan Boopathi; Cristiano Gomes; Stephen A Zderic; Bruce Malkowicz; Ranjita Chakrabarti; Darshan P Patel; Alan J Wein; Samuel Chacko Journal: Am J Physiol Cell Physiol Date: 2014-07-16 Impact factor: 4.249