From embryonic stem cells to blastema and MRL mice.

New scientific knowledge offers fresh opportunities for regenerative medicine and tissue repair. Among various clinical options, multipotent embryonic stem cells (ESC) prepared from inner cell masses of rabbit blastocysts have been tested over many years. More recently, stem cells have been isolated from individual tissues and from umbilical cord blood. These methods seemingly offer similar rates of repair and avoid ethical complexities arising from the need for human embryos to prepare ESC. Different methods of regenerating tissues have now emerged, based on the well-known forms of organ regeneration in urodeles such as salamanders. These methods depend on the formation of a blastema, and recent studies on MRL mice have revealed that they possess similar methods of repair as in salamanders. There is also some evidence showing that this form of repair is also active in human fetuses but not in adults. Detailed knowledge of these various forms of tissue repair is now urgently needed in order to assess the benefits of each form of treatment. These matters are discussed at the end of this review where various investigations clarify the benefits and drawbacks of these varied approaches to tissue repair.