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Literature DB >> 1833723

Rapid determination of nucleotide content and its application to the study of genome structure.

Abstract

We have developed a sensitive, reliable and accurate procedure for estimating the base composition of small samples of DNAs. This method has been applied to the analysis of genomic DNAs from several sources including large regions of human DNA cloned as yeast artificial chromosomes. To determine whether the human genome is compartmentalized into large segments of homogeneous base composition, we examined the GC content of a 1.2 megabase contig spanning the cystic fibrosis gene.

Entities: Disease Species

Mesh：

Substances：

Year: 1991 PMID： 1833723 PMCID： PMC328873 DOI： 10.1093/nar/19.19.5181

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

25 in total

1. Chromosomal region of the cystic fibrosis gene in yeast artificial chromosomes: a model for human genome mapping.

Authors: E D Green; M V Olson
Journal: Science Date: 1990-10-05 Impact factor: 47.728

2. Giant G+C% mosaic structures of the human genome found by arrangement of GenBank human DNA sequences according to genetic positions.

Authors: T Ikemura; K Wada; S Aota
Journal: Genomics Date: 1990-10 Impact factor: 5.736

3. The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion.

Authors: C Huxley; Y Hagino; D Schlessinger; M V Olson
Journal: Genomics Date: 1991-04 Impact factor: 5.736

4. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl.

Authors: C L SCHILDKRAUT; J MARMUR; P DOTY
Journal: J Mol Biol Date: 1962-06 Impact factor: 5.469

5. Selective staining with two fluorochromes of DNA fragments on gels depending on their AT content.

Authors: T Mabuchi; S Nishikawa
Journal: Nucleic Acids Res Date: 1990-12-25 Impact factor: 16.971

6. The compositional properties of human genes.

Authors: B Aïssani; G D'Onofrio; D Mouchiroud; K Gardiner; C Gautier; G Bernardi
Journal: J Mol Evol Date: 1991-06 Impact factor: 2.395

Review 7. CpG-rich islands and the function of DNA methylation.

Authors: A P Bird
Journal: Nature Date: 1986 May 15-21 Impact factor: 49.962

Review 8. The isochore organization of the human genome.

Authors: G Bernardi
Journal: Annu Rev Genet Date: 1989 Impact factor: 16.830

9. Fractions of HeLa DNA differing in their content of guanine+cytosine.

Authors: C L Schildkraut; J J Maio
Journal: J Mol Biol Date: 1969-12-14 Impact factor: 5.469

10. Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) gene.

Authors: J Zielenski; R Rozmahel; D Bozon; B Kerem; Z Grzelczak; J R Riordan; J Rommens; L C Tsui
Journal: Genomics Date: 1991-05 Impact factor: 5.736

3 in total

1. A rapid procedure for the compositional analysis of yeast artificial chromosomes.

Authors: A De Sario; E M Geigl; G Bernardi
Journal: Nucleic Acids Res Date: 1995-10-11 Impact factor: 16.971

2. Molecular, functional, and evolutionary analysis of sequences specific to Salmonella.

Authors: E A Groisman; M A Sturmoski; F R Solomon; R Lin; H Ochman
Journal: Proc Natl Acad Sci U S A Date: 1993-02-01 Impact factor: 11.205

3. A compositional map of human chromosome band Xq28.

Authors: A De Sario; E M Geigl; G Palmieri; M D'Urso; G Bernardi
Journal: Proc Natl Acad Sci U S A Date: 1996-02-06 Impact factor: 11.205

3 in total