Zheng-Jiang Cheng1, Li-Hua Hu, Shao-Jun Huang. 1. Department of Clinical Laboratory Medicine, Xiangfan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Xiangfan, Hubei, 441021, P. R. China.
Abstract
BACKGROUND & OBJECTIVE: Survivin, a bifunctional protein that regulates cell division and suppresses apoptosis, may play an important role in tumorigenesis. This study was to determine the correlations of survivin gene 31-GC polymorphisms to the occurrence of gastric carcinoma and survivin mRNA expression. METHODS: The -31G/C single nucleotide polymorphism (SNP) of survivin promoter in peripheral blood samples from 96 gastric carcinoma patients and 67 healthy subjects was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing. Survivin mRNA expression in gastric carcinoma tissues was detected by real-time quantitative reverse transcription-polymerase chain reaction (RQ-RT-PCR). RESULTS: The genotype frequencies for -31G/G, -31G/C and -31C/C were 20.84%, 39.58% and 39.58% in gastric carcinoma patients, and 46.26%, 41.80% and 11.94% in healthy subjects, respectively. The frequencies of survivin-31C allele and C/C genotype were significantly higher in gastric carcinoma patients than in healthy subjects [59.37% vs. 32.84%, Chi2 = 22.26, P<0.005, odds ratio (OR)=2.98, 95% confidence interval (CI)=1.96-4.51; 39.58% vs. 11.94%, Chi2 =14.88, P<0.005, OR=4.83, 95% CI=2.91-8.03], but survivin mRNA was overexpressed with no significant difference in gastric cancer tissues subgrouped by genotypes. CONCLUSION: The -31C genotype of survivin promoter is associated with gastric cancer, and may be a risk factor of gastric carcinoma.
BACKGROUND & OBJECTIVE: Survivin, a bifunctional protein that regulates cell division and suppresses apoptosis, may play an important role in tumorigenesis. This study was to determine the correlations of survivin gene 31-GC polymorphisms to the occurrence of gastric carcinoma and survivin mRNA expression. METHODS: The -31G/C single nucleotide polymorphism (SNP) of survivin promoter in peripheral blood samples from 96 gastric carcinomapatients and 67 healthy subjects was analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing. Survivin mRNA expression in gastric carcinoma tissues was detected by real-time quantitative reverse transcription-polymerase chain reaction (RQ-RT-PCR). RESULTS: The genotype frequencies for -31G/G, -31G/C and -31C/C were 20.84%, 39.58% and 39.58% in gastric carcinomapatients, and 46.26%, 41.80% and 11.94% in healthy subjects, respectively. The frequencies of survivin-31C allele and C/C genotype were significantly higher in gastric carcinomapatients than in healthy subjects [59.37% vs. 32.84%, Chi2 = 22.26, P<0.005, odds ratio (OR)=2.98, 95% confidence interval (CI)=1.96-4.51; 39.58% vs. 11.94%, Chi2 =14.88, P<0.005, OR=4.83, 95% CI=2.91-8.03], but survivin mRNA was overexpressed with no significant difference in gastric cancer tissues subgrouped by genotypes. CONCLUSION: The -31C genotype of survivin promoter is associated with gastric cancer, and may be a risk factor of gastric carcinoma.
Authors: Salvatore de Maria; Lorenzo Lo Muzio; Alessandra Braca; Paolo Rega; Amalia Cassano; Angela Vinella; Ruggiero Fumarulo; Rosario Serpico; Ernesto Farina; Vittoria Metafora; Giuseppe Pannone; Gian Pietro Ravagnan; Salvatore Metafora; Corrado Rubini; Maria Carteni; Maria Addolorata Mariggiò Journal: Oncol Lett Date: 2011-07-05 Impact factor: 2.967