Literature DB >> 18331041

Two-photon fluorescent turn-on probe for lipid rafts in live cell and tissue.

Hwan Myung Kim1, Byeong Ha Jeong, Ju-Yong Hyon, Myoung Jin An, Mun Sik Seo, Jin Hee Hong, Kyoung J Lee, Chul Hoon Kim, Taiha Joo, Seok-Cheol Hong, Bong Rae Cho.   

Abstract

We report a new two-photon fluorescence turn-on probe 6-[(E)-3-oxo-1-dodecenyl]-2-[N-methyl-N-(carboxymethyl)amino]naphthalene (CL2) that is designed specifically for visualizing lipid rafts in living cells and tissues. This probe emits much brighter two-photon excited fluorescence in lipid rafts than in non-raft domains and allows direct visualization of the lipid rafts in the live cells and pyramidal neuron layer of the CA1 region at a depth of 100-250 mum in live tissues using two-photon microscopy.

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Year:  2008        PMID： 18331041     DOI： 10.1021/ja711391f

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  19 in total

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