AIM: To investigate a possible increase of basolateral expression of carcinoembryonic antigen (CEA) by interfering with the apical transport machinery, we studied the effect of cholesterol depletion on CEA sorting and secretion. METHODS: Cholesterol depletion was performed in polarized Caco-2 cells using lovastatin and methyl-beta-cyclodextrin. RESULTS: We show that CEA is predominantly expressed and secreted at the apical surface. Reduction of the cholesterol level of the cell by 40%-50% with lovastatin and methyl-beta-cyclodextrin led to a significant change of the apical-to-basolateral transport ratio towards the basolateral membrane. CONCLUSION: As basolateral expression of CEA has been suggested to have anti-inflamatory properties, Cholesterol depletion of enterocytes might be a potential approach to influence the course of inflammatory bowel disease.
AIM: To investigate a possible increase of basolateral expression of carcinoembryonic antigen (CEA) by interfering with the apical transport machinery, we studied the effect of cholesterol depletion on CEA sorting and secretion. METHODS:Cholesterol depletion was performed in polarized Caco-2 cells using lovastatin and methyl-beta-cyclodextrin. RESULTS: We show that CEA is predominantly expressed and secreted at the apical surface. Reduction of the cholesterol level of the cell by 40%-50% with lovastatin and methyl-beta-cyclodextrin led to a significant change of the apical-to-basolateral transport ratio towards the basolateral membrane. CONCLUSION: As basolateral expression of CEA has been suggested to have anti-inflamatory properties, Cholesterol depletion of enterocytes might be a potential approach to influence the course of inflammatory bowel disease.
Authors: E P Kilsdonk; P G Yancey; G W Stoudt; F W Bangerter; W J Johnson; M C Phillips; G H Rothblat Journal: J Biol Chem Date: 1995-07-21 Impact factor: 5.157
Authors: A W Alberts; J Chen; G Kuron; V Hunt; J Huff; C Hoffman; J Rothrock; M Lopez; H Joshua; E Harris; A Patchett; R Monaghan; S Currie; E Stapley; G Albers-Schonberg; O Hensens; J Hirshfield; K Hoogsteen; J Liesch; J Springer Journal: Proc Natl Acad Sci U S A Date: 1980-07 Impact factor: 11.205
Authors: Julia Krammer; Margarete Digel; Friedrich Ehehalt; Wolfgang Stremmel; Joachim Füllekrug; Robert Ehehalt Journal: Int J Med Sci Date: 2011-10-07 Impact factor: 3.738